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D8062

DMEM/F-12

With ʟ-glutamine, sodium bicarbonate and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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Pack SizeSKUAvailabilityPrice
500 mL
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$60.00
6 x 500 mL
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$293.00

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
MDL number:
MFCD00217417

$60.00

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Product Name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

shipped in

ambient

storage temp.

2-8°C

General description

Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.

Application

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used:
  • to maintain human lung and colorectal cancer cell lines for the biological evaluation of the secondary metabolites
  • to culture human kidney-2 (HK-2) cells and as a component of the assay medium for lysophosphatidylcholine (LPC) treatment[1]
  • to culture mouse squamous cell carcinoma (SCCVII) tumor cells[2]

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This Item
D8437D6421D8900
Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D8062

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D8437

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham With 15 mM HEPES and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

D6421

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

Sigma-Aldrich

Sigma-Aldrich

D8900

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

glucose: 3.15 g/L
NaHCO3: yes
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: 0.055 g/L

components

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

components

sodium pyruvate: 0.055 g/L
L-glutamine: no
phenol red: yes
NaHCO3: yes
HEPES: 15 mM
glucose: 3.15 g/L

components

sodium pyruvate: 0.055 g/L
HEPES: 15 mM
NaHCO3: no
L-glutamine: yes
glucose: 3.15 g/L
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBL6949
RNBL5294
RNBL2018
RNBK9603
RNBK6544

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Hayato Fukusumi et al.
PeerJ, 6, e4187-e4187 (2018-01-10)
Since the development of human-induced pluripotent stem cells (hiPSCs), various types of hiPSC-derived cells have been established for regenerative medicine and drug development. Neural stem/progenitor cells (NSPCs) derived from hiPSCs (hiPSC-NSPCs) have shown benefits for regenerative therapy of the central
Tijmen H Booij et al.
SLAS discovery : advancing life sciences R & D, 22(8), 974-984 (2017-06-24)
Polycystic kidney disease (PKD) is a prevalent disorder characterized by renal cysts that lead to kidney failure. Various signaling pathways have been targeted to stop disease progression, but most interventions still focus on alleviating PKD-associated symptoms. The mechanistic complexity of
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Global Trade Item Number

SKUGTIN
D8062-6X500ML04061833589182
D8062-500ML04061833589175

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