According to the Enzymatic Assay Test Procedure, a solution should be prepared immediately before use, containing 500–1000 Kunitz units/ml in cold deionized water. More information can be found in the document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/769/284/d8764enz.pdf
D8764
Deoxyribonuclease II from bovine spleen
Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein
Synonyme(s) :
DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase
pour consulter les tarifs organisationnels et contractuels.
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
30000 units | Vérifier la disponibilité des articles du panier | 128,00 € |
150000 units | Disponible dès aujourd'huiDeSt QuentinFallavier | 369,00 € |
300000 units | Vérifier la disponibilité des articles du panier | 629,00 € |
A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
Specific activity:
≥1,000 units/mg protein
Biological source:
bovine spleen
Concentration:
≥50% protein
128,00 €
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Laissez-nous vous aiderbiological source
bovine spleen
Quality Segment
type
Type V
form
essentially salt-free, lyophilized powder
specific activity
≥1,000 units/mg protein
mol wt
38 kDa
concentration
≥50% protein
technique(s)
DNA extraction: suitable, tissue culture: suitable
input
general use null
suitability
suitable for molecular biology
application(s)
cell analysis
foreign activity
RNase ≤0.1%
storage temp.
−20°C
Application
Deoxyribonuclease II from bovine spleen has been used:
Biochem/physiol Actions
Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.[5]
Other Notes
One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM
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Cet article | |||
|---|---|---|---|
| technique(s) DNA extraction: suitable, tissue culture: suitable | technique(s) - | technique(s) DNA purification: suitable | technique(s) DNA extraction: suitable |
| biological source bovine spleen | biological source - | biological source bovine pancreas | biological source bovine |
| specific activity ≥1,000 units/mg protein | specific activity 2,000-6,000 Kunitz units/mg protein (biuret) | specific activity ≥2,000 units/mg protein | specific activity ≥4,000 units/mg protein |
| form essentially salt-free, lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| suitability suitable for molecular biology | suitability - | suitability suitable for molecular biology | suitability suitable for molecular biology |
| application(s) cell analysis | application(s) - | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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What is the best buffer to dissolve the lyophilized powder in? What is the best final concentration to be prepared?
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