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D8764

Deoxyribonuclease II from bovine spleen

Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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30000 units
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1 410,00 kr
150000 units
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4 110,00 kr
300000 units
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7 020,00 kr

About This Item

CAS Number:
9025-64-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-801-8
MDL number:
MFCD00130919
EC Number:
3.1.22.1 (BRENDA, IUBMB)
Specific activity:
≥1,000 units/mg protein
Biological source:
bovine spleen
Concentration:
≥50% protein

1 410,00 kr

Please contact Customer Service for Availability
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biological source

bovine spleen

Quality Level

200

type

Type V

form

essentially salt-free, lyophilized powder

specific activity

≥1,000 units/mg protein

mol wt

38 kDa

concentration

≥50% protein

technique(s)

DNA extraction: suitable, tissue culture: suitable

suitability

suitable for molecular biology

application(s)

cell analysis

foreign activity

RNase ≤0.1%

storage temp.

−20°C

Application

Deoxyribonuclease II from bovine spleen has been used:
  • as a component of digestion mix to isolate immune cells from colon samples[1]
  • to digest retinal explants for single-cell suspension preparation[2]
  • for isolation of stromal cells from human colon tissue[3]
  • in the dissociation medium during the preparation of embryonic cardiac myocytes from rat heart[4]

Biochem/physiol Actions

Deoxyribonuclease II, also called as acid DNAse, hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3′-phosphates. in vitro, its optimum pH range is 4.5 - 5.0. It also acts upon p-nitrophenyl-phosphodiesters at pH 5.6 - 5.9. The molecular weight is approximately 38,000 Da.[5]

Other Notes

One Kunitz unit will produce a ΔA260 of 0.001 per min per mL at pH 4.6 at 25 °C; [Mg2+] = 0.83 mM

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This Item
D4138D4513D2821
Deoxyribonuclease II from bovine spleen Type V, essentially salt-free, lyophilized powder, ≥1,000 units/mg protein

Sigma-Aldrich

D8764

Deoxyribonuclease II from bovine spleen

Deoxyribonuclease II from porcine spleen Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Sigma-Aldrich

D4138

Deoxyribonuclease II from porcine spleen

Deoxyribonuclease I from bovine pancreas Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein

Sigma-Aldrich

D4513

Deoxyribonuclease I from bovine pancreas

Deoxyribonuclease I bovine recombinant, expressed in Pichia pastoris, lyophilized powder, RNAse and protease, free

Sigma-Aldrich

D2821

Deoxyribonuclease I bovine

specific activity

≥1,000 units/mg protein

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

specific activity

≥2,000 units/mg protein

specific activity

≥4,000 units/mg protein

biological source

bovine spleen

biological source

-

biological source

bovine pancreas

biological source

bovine

technique(s)

DNA extraction: suitable, tissue culture: suitable

technique(s)

-

technique(s)

DNA purification: suitable

technique(s)

DNA extraction: suitable

application(s)

cell analysis

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

concentration

≥50% protein

concentration

-

concentration

-

concentration

-

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000545295
0000545296
0000545295
0000545296
0000500435

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Related Content

K Tempel
Arzneimittel-Forschung, 30(7), 1119-1123 (1980-01-01)
The influence of three mucopolysaccharide-polysulfuric acid-esters (MPS) of different molecular weight on DNAase II-activity as well as on nucleic acid and protein synthesis of lymphocytes of rat spleen has been investigated in vitro. The results are summarized as follows: 1.
Dopamine D2 receptor stimulation differentially affects voltage-activated calcium channels in rat pituitary melanotropic cells.
Keja J A, et al.
The Journal of Physiology, 450(1), 409-435 (1992)
S Ingebrandt et al.
Biosensors & bioelectronics, 16(7-8), 565-570 (2001-09-07)
An extracellular recording system has been designed for the detection of electrical cell signals using p-channel or n-channel field-effect transistor (FET) arrays with non-metallized gates. Signals from rat heart muscle cell were recorded by these devices and the results described
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Global Trade Item Number

SKUGTIN
D8764-150KU04061832755489
D8764-300KU04061832755496
D8764-30KU04061832755502

Questions

  1. What is the best buffer to dissolve the lyophilized powder in? What is the best final concentration to be prepared?

    1 answer

    1. According to the Enzymatic Assay Test Procedure, a solution should be prepared immediately before use, containing 500–1000 Kunitz units/ml in cold deionized water. More information can be found in the document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/769/284/d8764enz.pdf

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