The specific salmon species is not determined for this product. See the link below to review a sample Certificate of Origin for this material:
https://www.sigmaaldrich.com/coo/undefined/D9156/SLBM7249V.
D9156
Deoxyribonucleic acid, single stranded from salmon testes
For hybridization
Synonym(s):
single-stranded template DNA
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Estimated to ship TODAYfromSAINT LOUIS | $99.10 |
5 mL | Estimated to ship TODAYfromSAINT LOUIS | $311.00 |
5 x 1 mL | Estimated to ship TODAYfromSAINT LOUIS | $345.00 |
About This Item
Assay:
9-11 mg/mL (DNA concentration)
Biological source:
fish testis (salmon)
Form:
solution
Storage temp.:
−20°C
$99.10
Estimated to ship TODAYDetails
biological source
fish testis (salmon)
Quality Segment
grade
Molecular Biology
assay
9-11 mg/mL (DNA concentration)
form
solution
concentration
10 mg/mL in H2O
shipped in
dry ice
storage temp.
−20°C
General description
A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .
Application
Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples and human blood monocyte subpopulations.
For use as a blocking agent in Northern and Southern blotting.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.
Preparation Note
This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
Other Notes
DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.
1 of 1
This Item | |||
|---|---|---|---|
| description For hybridization | description lyophilized powder | description - | description Type XV, Activated, lyophilized powder |
| grade Molecular Biology | grade Molecular Biology | grade - | grade Molecular Biology |
| biological source fish testis (salmon) | biological source bovine (calf) thymus | biological source - | biological source bovine pancreas |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| assay 9-11 mg/mL (DNA concentration) | assay - | assay - | assay - |
| form solution | form lyophilized powder | form solid | form lyophilized powder |
| concentration 10 mg/mL in H2O | concentration - | concentration - | concentration - |
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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