Skip to Content
MilliporeSigma

Key Documents

View All Documentation

Skip To

E0885

Enterokinase from porcine intestine

lyophilized powder, ≥100 units/mg protein

Synonym(s):

Enteropeptidase

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Pack SizeSKUAvailabilityPrice
40 units
Please contact Customer Service for Availability
$411.00
200 units
Please contact Customer Service for Availability
$1,670.00

About This Item

CAS Number:
9014-74-8
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
MFCD00131020
EC Number:
3.4.21.9 (BRENDA, IUBMB)
Specific activity:
≥100 units/mg protein

$411.00

Please contact Customer Service for Availability
​
Request a Bulk Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

lyophilized powder

Quality Segment

200

specific activity

≥100 units/mg protein

mol wt

150 kDa

purified by

chromatography

composition

Protein, ≥20% Lowry

foreign activity

aminopeptidase ≤1.5%, trypsin ≤1%, free

storage temp.

−20°C

Application

Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide.
The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen.[1] The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus[2] The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa.[3] It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.

Biochem/physiol Actions

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

Physical form

Lyophilized powder containing sodium phosphate buffer salts

Other Notes

One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
E0632E0127C9584
Enterokinase from porcine intestine lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

E0885

Enterokinase from porcine intestine

Enterokinase from porcine intestine ≥0.5 units/mg solid

Sigma-Aldrich

E0632

Enterokinase from porcine intestine

Elastase from porcine pancreas Type III, lyophilized powder, Protein 55-85 %, ≥4.0 units/mg protein

Sigma-Aldrich

E0127

Elastase from porcine pancreas

Carboxypeptidase B from porcine pancreas lyophilized powder

Sigma-Aldrich

C9584

Carboxypeptidase B from porcine pancreas

specific activity

≥100 units/mg protein

specific activity

≥0.5 units/mg solid

specific activity

≥4.0 units/mg protein

specific activity

≥125 units/mg protein

form

lyophilized powder

form

salt-free, lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

150 kDa

mol wt

150 kDa

mol wt

25.9 kDa

mol wt

34,000 Da± 600

foreign activity

aminopeptidase ≤1.5%, trypsin ≤1%, free

foreign activity

-

foreign activity

-

foreign activity

carboxypeptidase A ≤1%, chymotrypsin ≤0.1%, trypsin ≤5%

purified by

chromatography

purified by

-

purified by

-

purified by

affinity chromatography

Still not finding the right product?

Explore all of our products under Enterokinase from porcine intestine

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000563910
0000563911
0000538922
0000530320
0000545387

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Related Content

Global Trade Item Number

SKUGTIN
E0885-200UN04061833601273
E0885-40UN04061833601280

Questions

Reviews

No rating value

Active Filters