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E1250

Elastase from porcine pancreas

Name: Elastase from porcine pancreas
Brand: SIGMA
Price: 86.6 CAD

Type I, ≥4.0 units/mg protein

Synonyme(s) :

Elastase from hog pancreas, Pancreatopeptidase E

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À vous/Référence	Disponibilité	Prix
10 mg	Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.	86,60 $
25 mg	Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.	147,00 $
50 mg	Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.	260,00 $
100 mg	Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.	434,00 $
500 mg	Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.	1 800,00 $

A propos de cet article

Numéro CAS:

39445-21-1

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

254-453-6

MDL number:

MFCD00130998

Numéro CE :

3.4.21.36 (BRENDA, IUBMB)

Specific activity:

≥4.0 units/mg protein

Biological source:

Porcine pancreas

Concentration:

0.5-15.0 mg/mL in water

86,60 $

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Propriétés

biological source

Porcine pancreas

Quality Segment

200

type

Type I

form

suspension

specific activity

≥4.0 units/mg protein

mol wt

25.9 kDa

contains

0.1% thymol

concentration

0.5-15.0 mg/mL in water

foreign activity

trypsin ≤50 BAEE units/mg protein

storage temp.

2-8°C

Description

Application

Elastase from porcine pancreas has been used:

to induce abdominal aortic aneurysm (AAA)
to study the impact of indoleamine 2-3 dioxygenase 1 (IDO) in mice
to digest aortas for aortic smooth muscle cells (SMC) isolation
as a positive control of proteolytic digestion

The enzyme from Sigma has been used in the development of elastase-perfused animal model . This study determined if tobacco exposure could lower the threshold of aortic injury necessary for AAA (abdominal aortic aneurysm) development. It has also been used during the isolation of type II pneumocytes from human lungs.

Elastase from porcine pancreas has been used in a study to investigate the design, synthesis and evaluation of biomimetic affinity ligands for elastases. Elastase from porcine pancreas has also been used in a study to investigate the purification and partial characterization of the pancreatic proteolytic enzymes trypsin, chymotrypsin, and elastase.

Biochem/physiol Actions

Elastase hydrolyses elastin, the specific protein of elastic fibers, and digests hemoglobin, casein and fibrin.

Elastase is a single polypeptide chain of 240 amino acid residues and contains four disulfide bridges. The molecular mass is approximately 25.9 kDa. The enzyme is synthesized as an inactive zymogen, proelastase, which is converted to the active form by limited proteolysis at the N-terminal by trypsin. It is a serine protease with broad specificity. It cleaves protein at the carboxyl side of small hydrophobic amino acids such as Ile, Gly, Ala, Ser, Val, and Leu. The enzyme also hydrolyzes amides and esters such as N-Benzoyl-L-alanine methyl ester. The pH optimum is found to be 8.0-8.5. It does not require any activator, but it is inhibited by diisopropyl fluorophosphate, phenylmethanesulfonyl fluoride, α2-macroglobulin, α1-antitrypsin, sulfonyl fluorides and p-dinitrophenyl diethylphosphate and high salt concentrations. It is extensively used in tissue and cell dissociation procedures. Elastase is effective in the isolation of Type II lung cells.

Packaging

Package size based on protein content

Preparation Note

2× crystallized

Other Notes

One unit will hydrolyze 1.0 μmole of N-succinyl-L-Ala-Ala-Ala-p-nitroanilide per min, pH 8.0 at 25 °C.

Comparer avec des articles similaires

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1 of 1

Cet article	E0258	E7885	324682
Sigma-Aldrich E1250 Elastase from porcine pancreas	Sigma-Aldrich E0258 Elastase from porcine pancreas	Sigma-Aldrich E7885 Elastase from porcine pancreas	Sigma-Aldrich 324682 Elastase, Porcine Pancreas, High Purity, Crystallized
description Type I, ≥4.0 units/mg protein	description Type IV, Protein 50-90 %, lyophilized powder, ≥4.0 units/mg protein (biuret)	description lyophilized powder, suitable for cell culture	description Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.
biological source Porcine pancreas	biological source Porcine pancreas	biological source Porcine	biological source -
specific activity ≥4.0 units/mg protein	specific activity ≥4.0 units/mg protein (biuret)	specific activity ≥4 units/mg protein	specific activity ≥25 units/mg protein
concentration 0.5-15.0 mg/mL in water	concentration -	concentration -	concentration -
form suspension	form lyophilized powder	form lyophilized powder	form lyophilized
mol wt 25.9 kDa	mol wt -	mol wt 25.9 kDa	mol wt -
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

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pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Classe de stockage

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Questions

Anonymous

2 years ago

What is the difference between Type I and Type IV Elastase?

1 answer
1. Technical Support
  
  2 years ago
  
  The major differences between the different types of Elastase are as follows:
  E1250: Type I, aqueous suspension, >4 units/mg protein, <50 units/mg protein Trypsin. Contains Thymol as an antimicrobial, which is toxic to cells.
  E6883: Type IIA, discontinued
  E0127: Type III, lyophilized powder, >4 units/mg protein, <200 units/mg protein Trypsin.
  E0258: Type IV, lyophilized powder, >4 units/mg protein, <50 units/mg protein Trypsin, further purification of E0127.
  E7885: Bioreagent, lyophilized powder, suitability tested for use in cell culture, >4 units/mg protein, <50 units/mg protein Trypsin.
  45125: Lyophilized powder, >6.4 units/mg protein, not tested for Trypsin impurity.
  
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