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E1385

Ethidium bromide solution

BioReagent, Molecular Biology, 500 μg/mL in H2O

Synonym(s):

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

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Pack SizeSKUAvailabilityPrice
5 mL

Available to ship TODAYfromMILWAUKEE

$39.20

About This Item

Empirical Formula (Hill Notation):
C21H20BrN3
CAS Number:
1239-45-8
Molecular Weight:
394.31
UNSPSC Code:
41105322
NACRES:
NA.31
PubChem Substance ID:
24894408
MDL number:
MFCD00011724
Beilstein/REAXYS Number:
3642536

$39.20

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grade

Molecular Biology

Quality Level

200

product line

BioReagent

concentration

500 μg/mL in H2O

technique(s)

electrophoresis: suitable

suitability

suitable for gel electrophoresis

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

Application

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Ethidium bromide solution has been used to deplete mtDNA to investigate mitochondrial damage signals in autophagy. It has also been used to detect DNA damage using comet assay.

Biochem/physiol Actions

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Preparation Note

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

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Ethidium bromide solution BioReagent, Molecular Biology, 500 μg/mL in H2O

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Ethidium bromide solution BioReagent, Molecular Biology, 10 mg/mL in H2O

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E1510

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Ethidium bromide solution suitable for fluorescence, ~1% in H2O

Sigma-Aldrich

46067

Ethidium bromide solution

Ethidium bromide solution 10 mg/mL

Sigma-Aldrich

09-0617

Ethidium bromide solution

grade

Molecular Biology

grade

Molecular Biology

grade

-

grade

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

suitability

suitable for gel electrophoresis

suitability

suitable for gel electrophoresis

suitability

-

suitability

-

concentration

500 μg/mL in H2O

concentration

10 mg/mL in H2O

concentration

~1% in H2O

concentration

10 mg/mL

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

product line

BioReagent

product line

BioReagent

product line

-

product line

-

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT5701
SHBR2368
SHBR0113
SHBQ1937
SHBN4521

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Related Content

Ethidium Bromide

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

Product Information Sheet - E1385

Product Information Sheet

Hydrogen peroxide-induced oxidative damage in peripheral blood lymphocytes from rats chronically treated with corticosterone: The protective effect of oxytocin treatment.
Stanic D
Chemico-Biological Interactions, 256, 134-141 (2016)
Autophagy-deficient Kupffer cells promote tumorigenesis by enhancing mtROS-NF-kappaB-IL1alphabeta-dependent inflammation and fibrosis during the preneoplastic stage of hepatocarcinogenesis
Sun K, et al.
Cancer letters, 388, 198-207 (2017)
Ulrich Braunschweig et al.
Genome research, 24(11), 1774-1786 (2014-09-27)
Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is
View All Related Papers

Global Trade Item Number

SKUGTIN
E1385-5ML04061838255266

Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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