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β-Endothelial Cell Growth Factor human

Name: &#946;-Endothelial Cell Growth Factor human
Brand: SIGMA
Price: 427 USD
Availability: InStock

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

ECGS, β-ECGF

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Size/SKU	Availability	Price
25 μg	Available to ship TODAYfromMILWAUKEE	$427.00

About This Item

CAS Number:

105843-51-4

UNSPSC Code:

12352202

NACRES:

NA.77

MDL number:

MFCD00131012

Biological source:

human

Recombinant:

expressed in E. coli

Assay:

≥97% (SDS-PAGE)

Form:

lyophilized powder

Mol wt:

predicted mol wt ~17 kDa

Impurities:

≤1.0 EU/μg protein Endotoxin level (LAL test)

$427.00

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Properties

Product Name

β-Endothelial Cell Growth Factor human, β-ECGF, recombinant, expressed in E. coli, suitable for cell culture

biological source

human

Quality Segment

200

recombinant

expressed in E. coli

assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.03-0.3 ng/mL ED₅₀/EC₅₀

quality

endotoxin tested

mol wt

predicted mol wt ~17 kDa

packaging

pkg of 25 μg

storage condition

avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

technique(s)

cell culture | mammalian: suitable

impurities

≤1.0 EU/μg protein Endotoxin level (LAL test)

UniProt accession no.

P49765

storage temp.

−20°C

Gene Information

human ... VEGFB(7423)

Description

General description

β‑Endothelial Cell Growth Factor (β‑ECGF) is a recombinant human protein expressed in Escherichia coli and supplied as a lyophilized powder suitable for mammalian cell culture. The β‑ECGF gene encodes an endothelial growth factor and is mapped to human chromosome 11q13.1. Recombinant β‑ECGF consists of a 14‑amino‑acid N‑terminal extension of acidic fibroblast growth factor. After removal of the initiation methionine, the protein comprises 154 amino acids with a predicted molecular weight of approximately 17 kDa.

Application

Recombinant human β‑Endothelial Cell Growth Factor (β‑ECGF) has been used in a range of endothelial research applications, including:

Endothelial cell culture studies
Human umbilical vein endothelial cell (HUVEC) culture
Cell death and viability assays in in vitro model systems

Biochem/physiol Actions

β‑Endothelial Cell Growth Factor (β‑ECGF) is a potent mitogen for endothelial cells, with mitogenic activity reported to be enhanced in the presence of heparin. In vitro, β‑ECGF stimulates the growth of quiescent human umbilical vein endothelial cells (HUVECs). β‑ECGF has been associated with endothelial‑mediated lipid and glucose metabolism pathways and has been studied extensively in cellular and molecular research models.

Physical form

Lyophilized from a 0.2 μm filtered solution in 10 mM MOPS, 50 mM ammonium sulfate, 0.2 mM DTT, and 0.2 mM EDTA, pH 7.2, containing 50 μg bovine serum albumin per 1 μg cytokine.

Analysis Note

The proliferative activity is tested in culture by using quiescent NR6R-3T3 fibroblasts.

Other Notes

14 amino acid, N-terminal extended form of human FGF-acidic.

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This Item	H8541	T5425	F5542
Sigma-Aldrich E1388 β-Endothelial Cell Growth Factor human	Sigma-Aldrich H8541 Transforming Growth Factor-β1 human	Sigma-Aldrich T5425 Transforming Growth Factor-β3 human	Sigma-Aldrich F5542 Fibroblast Growth Factor-Acidic human
description β-ECGF, recombinant, expressed in E. coli, suitable for cell culture	description TGF-β1, Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture	description TGF-β3, recombinant, expressed in baculovirus infected Sf21 cells, powder, suitable for cell culture	description FGF-Acidic, recombinant, expressed in E. coli, suitable for cell culture
assay ≥97% (SDS-PAGE)	assay ≥95% (SDS-PAGE)	assay ≥97% (SDS-PAGE)	assay ≥97% (SDS-PAGE)
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source human	biological source human	biological source human	biological source human
recombinant expressed in E. coli	recombinant expressed in HEK 293 cells	recombinant expressed in baculovirus infected Sf21 cells	recombinant expressed in E. coli
Quality Level 200	Quality Level 300	Quality Level 100, 200	Quality Level 200
form lyophilized powder	form lyophilized powder	form powder	form lyophilized powder

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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