This product is intended for research use only. It is not suitable for human or veterinary use. The material has not been tested for in vivo use. The end user would need to determine suitability. Solutions intended for use in animal models would require sterile filtration prior to administration.
E2257
β-Estradiol
powder, γ-irradiated, suitable for cell culture
Synonym(s):
1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mg | Check Cart for Availability | $108.00 |
About This Item
Empirical Formula (Hill Notation):
C18H24O2
CAS Number:
Molecular Weight:
272.38
UNSPSC Code:
12352209
NACRES:
NA.77
PubChem Substance ID:
EC Number:
200-023-8
Beilstein/REAXYS Number:
1914275
MDL number:
Biological source:
synthetic (organic)
Form:
powder
Assay:
≥98% (HPLC)
biological source
synthetic (organic)
Quality Level
sterility
γ-irradiated
product line
BioXtra
assay
≥98% (HPLC)
form
powder
technique(s)
cell culture | mammalian: suitable
mp
176-180 °C (lit.)
solubility
ethanol: 1 mg/mL, clear, colorless to very faintly yellow
suitability
suitable for
shipped in
ambient
storage temp.
room temp
SMILES string
O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
InChI
1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
InChI key
VOXZDWNPVJITMN-ZBRFXRBCSA-N
Gene Information
human ... ESR1(2099), ESR2(2100), ESRRA(2101), ESRRB(2103), GPER(2852), SERPINA6(866)
mouse ... Esr1(13982), Esr2(13983), Esrra(26379)
rat ... Afp(24177), Ar(24208), Esr1(24890), Esr2(25149), Shbg(24775)
General description
β-estradiol is a natural human estrogen. It is important for the growth of breast and reproductive epithelia, maturation of long bones and the development of secondary sexual characteristics. Estradiol is produced by immature germ cells, spermatozoa, Leydig cells and Sertoli cells. It plays a crucial role in proliferation, regulation of ion transport and regulation of apoptosis in Sertoli cells. Estradiol regulates sperm concentration, motility and morphology.[1] β-estradiol functions as a menopausal hormone. It is implicated in replacement therapy for female hypogonadism or primary ovarian failure. Reduced levels of β-estradiol are associated with heart disease and osteoporosis.
Application
β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).
β-estradiol has been used:
- for primary culture of mouse endometrial stromal cells (ESCs)
- for estrogen treatment in vitro
- in the in vitro maturation of cumulus–oocyte complexes (COCs)
Biochem/physiol Actions
The major estrogen secreted by the premenopausal ovary.
The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.
Physical form
powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw
Preparation Note
To prepare 20 μg/ml stock solution: add 1 ml absolute ethanol; gently swirl to dissolve; add 49 ml sterile medium while mixing.
1 of 1
This Item | |||
|---|---|---|---|
| biological source synthetic (organic) | biological source synthetic (organic) | biological source - | biological source - |
| assay ≥98% (HPLC) | assay ≥98% (HPLC) | assay - | assay - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
| product line BioXtra | product line BioReagent | product line - | product line - |
| form powder | form powder | form - | form - |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, single cell analysis: suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) HPLC: suitable, gas chromatography (GC): suitable |
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signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Find documentation for the products that you have recently purchased in the Document Library.
Estrogen preserves Fas ligand levels by inhibiting microRNA-181a in bone marrow-derived mesenchymal stem cells to maintain bone remodeling balance
Shao B, et al.
Faseb Journal, 29(9), 3935-3944 (2015)
DHEA improves the antioxidant capacity of endometrial stromal cells and improves endometrium receptivity via androgen receptor
Qin A, et al.
Eur. J. Obstet. Gyn. Rep. Biol., 198, 120-126 (2016)
Improving in vitro maturation and pregnancy outcome in cattle using a novel oocyte shipping and maturation system not requiring a CO2 gas phase
Barcelo-Fimbres M, et al.
Theriogenology, 84(1), 109-117 (2015)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E2257-1MG | 04061833602553 |
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can this type estradiol be injected intraperitoneally in rat?
1 answer-
Helpful?
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What is the shelf life as a powder? What is the shelf life once in solution and stored at -20 degrees C?
1 answer-
This product does not contain an expiration date. It contains a recommended retest date of three years after the quality release date. A recommended retest date is the period of time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications. For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.
Upon resuspension, solutions can be stored frozen at -20°C for up to 3 months . It is advised to avoid repeated freeze/thaw cycles. If stored in the refrigerator (4°C), de-gassed stock solutions are stable for up to 6 months.
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