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E2520

Anti-Epidermal Growth Factor antibody, Mouse monoclonal

clone EGF-10, purified from hybridoma cell culture

Synonym(s):

Anti-CEGF

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0.2 mL

Available to ship TODAYfromMILWAUKEE

$848.00

About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00239669
Conjugate:
unconjugated
Clone:
EGF-10, monoclonal
Application:
ARR, DB, ELISA (i), IHC (p), Neutral, RIA, WB
Citations:
13

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biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

EGF-10, monoclonal

form

buffered aqueous solution

mol wt

antigen 6 kDa

species reactivity

human

technique(s)

dot blot: 0.1 μg/mL using recombinant human EGF, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using human tissue, indirect ELISA: suitable, microarray: suitable, neutralization: suitable, radioimmunoassay: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

P01133

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGF(1950)

General description

Monoclonal Anti-Epidermal Growth Factor (EGF) (mouse IgG1 isotype) is derived from the EGF-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Epidermal growth factor (EGF) is a single chain polypeptide hormone (6 kDa). EGF protein exists largely in a β-sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in α-granules of blood platelets.

Immunogen

recombinant human EGF

Application

Monoclonal Anti-Epidermal Growth Factor antibody has been used in dot-blot, radioimmunoassay, enzyme linked immunosorbent assay (ELISA), immunohistology, immunoblotting, immunostaining.
Monoclonal Anti-Epidermal Growth Factor antibody produced in mouse was used to study rat immune cells by flow cytometry to detect the presence or absence of hormone and hormone-like molecules in response to EGF treatment.

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor (EGF) is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization.
Specifically reacts with both native and recombinant human EGF. The antibody does not cross-react with mouse EGF.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
E2760F1672F6162
Anti-Epidermal Growth Factor antibody, Mouse monoclonal clone EGF-10, purified from hybridoma cell culture

Sigma-Aldrich

E2520

Anti-Epidermal Growth Factor antibody, Mouse monoclonal

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse clone 29.1, ascites fluid

Sigma-Aldrich

E2760

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

Anti-Fibroblast Growth Factor-9 antibody, Mouse monoclonal clone FG9-77, purified from hybridoma cell culture

Sigma-Aldrich

F1672

Anti-Fibroblast Growth Factor-9 antibody, Mouse monoclonal

Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal clone FB-8, purified from hybridoma cell culture

Sigma-Aldrich

F6162

Anti-Fibroblast Growth Factor-Basic antibody, Mouse monoclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

species reactivity

human

species reactivity

pig, human, rat, mouse, canine

species reactivity

mouse, human

species reactivity

bovine, human

clone

EGF-10, monoclonal

clone

29.1, monoclonal

clone

FG9-77, monoclonal

clone

FB-8, monoclonal

Gene Information

human ... EGF(1950)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

Gene Information

human ... FGF9(2254)
mouse ... Fgf9(14180)

Gene Information

human ... FGF2(2247)

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

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Certificates of Analysis (COA)

Lot/Batch Number
0000446958
0000446958
0000415138
0000415138
0000414763

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Global Trade Item Number

SKUGTIN
E2520-.2ML04061833602720

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