E4013
Monoclonal Anti-Elastin antibody produced in mouse
clone BA-4, ascites fluid
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 μL | Check Cart for Availability | $185.00 | |
| 100 μL | Check Cart for Availability | $505.00 |
About This Item
Conjugate:
unconjugated
Clone:
BA-4, monoclonal
Application:
ARR, IHC (p)
Citations:
29
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
BA-4, monoclonal
mol wt
antigen 67.5 kDa
contains
15 mM sodium azide
species reactivity
human, goat, sheep, guinea pig, pig, canine, feline, bovine
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:5,000 using sheep arterial tissue, microarray: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ELN(2006)
General description
Monoclonal Anti-Elastin (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Elastin is an important polymeric protein of connective tissue. It is synthesized and secreted as a soluble single-chain protein (tropoelastin) that undergoes numerous post-ribosomal modifications prior to the organization of the elastic fiber in the extracellular space.
Immunogen
bovine α-elastin.
Application
Monoclonal Anti-Elastin antibody produced in mouse has been used in:
- immunohistochemistry
- immunostaining
- immunoblotting
Biochem/physiol Actions
Elastin is encoded by the ELN gene in humans. It is a polymeric protein in connective tissue that has exceptional properties of extension, elastic recoil to the extracellular matrix and allows many tissues in the body to resume their shape after stretching or contracting. Elastin is a self-assembling, extracellular-matrix protein providing tissue elasticity and is predominantly comprised of cross-linked tropoelastin. It plays an important role in the atherosclerotic process. The conformational disorder is caused due to a constitutive feature of elastin structure and function.
Elastin undergoes changes of morphology with aging and in many disease states. For example, abnormal accumulation of connective tissue in blood vessels contributes to alterations in vascular physiology associated with diseases such as hypertension and atherosclerosis. Several heritable diseases such as Marfan syndrome, pseudoxanthoma elasticum and the Buschke-Ollendorf syndrome may result from molecular defects of elastin.
The antibody binds to insoluble elastin, α-elastin, tropoelastin (the soluble non-cross linked precursor of elastin) and to peptide fragments generated by proteolytic digestion of insoluble elastin. In tropoelastin preparations, three immunoreactive bands have been observed at 67.5 kDa, 65 kDa and 62 kDa corresponding to the three elastin isoforms. The antibody is specific for an epitope composed of the repeated sequence in elastin: Val-Gly-Val-Ala-Pro-Gly. This epitope has been shown to be a chemoattractant for fibroblasts and monocytes.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| species reactivity human, goat, sheep, guinea pig, pig, canine, feline, bovine | species reactivity marsupial (Ptoorous tridactylis PtK2 cell line), rat | species reactivity human, pig | species reactivity mouse, bovine, human, chicken |
| Gene Information human ... ELN(2006) | Gene Information rat ... Plec1(64204) | Gene Information chicken ... NEFM(396206) | Gene Information human ... ELN(2006) |
| antibody form ascites fluid | antibody form ascites fluid | antibody form ascites fluid | antibody form purified immunoglobulin |
| clone BA-4, monoclonal | clone 7A8, monoclonal | clone NN18, monoclonal | clone 10B8, monoclonal |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Related Content
Instructions
Fabricated elastin
Yeo GC, et al.
Advanced Helathcare Materials, 4(16), 2530-2530 (2015)
Chae-Eun Yang et al.
International journal of molecular sciences, 21(6) (2020-03-28)
An adenoviral vector (Ad) expressing a Wnt decoy receptor (sLRP6E1E2) is known to induce an anti-fibrotic effect by inhibiting Wnt signaling. We evaluated its effects in vivo using pig models and attempted to introduce an alginate gel-matrix system to prolong
Sean E Reichheld et al.
The Journal of biological chemistry, 289(14), 10057-10068 (2014-02-20)
Elastin is the intrinsically disordered polymeric protein imparting the exceptional properties of extension and elastic recoil to the extracellular matrix of most vertebrates. The monomeric precursor of elastin, tropoelastin, as well as polypeptides containing smaller subsets of the tropoelastin sequence
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E4013-100UL | 04061837883590 |
| E4013-.2ML | 04061837883583 |