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E5144

Enterokinase from bovine intestine

powder

Synonym(s):

Enteropeptidase

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Size/SKUAvailabilityPrice
50 units

Estimated to ship onSeptember 29, 2027fromSAINT LOUIS

$444.00

About This Item

CAS Number:
9014-74-8
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.56
MDL number:
MFCD00131020
EC Number:
3.4.21.9 (BRENDA, IUBMB)
Biological source:
bovine
Recombinant:
expressed in Pichia pastoris

$444.00

Estimated to ship onSeptember 29, 2027Details

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Quality Segment

biological source

bovine

recombinant

expressed in Pichia pastoris

form

powder

mol wt

42.3 kDa (+/- 6.4 kDa)

color

white

shipped in

dry ice

storage temp.

−20°C

General description

Enterokinase also referred to as enteropeptidase, is a transmembrane protein. The amino-terminal sequence of the bovine enteropeptidase is homologous to trypsin-like serine proteases. Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Application

Enterokinase from bovine intestine has been used to remove S-Tag and N-terminal His-tag from recombinant glutathione peroxidase 1 (Gpx1). It has also been used to cleave interleukin-2 (IL-2) protein from the granules
Enterokinase is a member of the S1 peptidase family. In vivo, it is responsble for the proteolytic activation of trypsin from trypsinogen. Enterokinase is used for site specific cleavage of recombinant fusion proteins containing an accessible enterokinase recognition site for removal of affinity tags.

Biochem/physiol Actions

Enterokinase catalyzes the proteolytic activation of pancreatic proteases. This action prevents the harmful tissue damage produced by the autoactivation of pancreatic proteases in the pancreas. Enterokinase recognizes Lys or Arg residues in the peptide. Lack of enterokinase can harm food digestion and absorption mechanism. Enterokinase is a highly specific serine protease that is used for the removal of the FLAG peptide from N-terminal and Met-N-terminal fusion proteins. It does not remove the C-terminal FLAG.

Packaging

Supplied with optimized enterokinase buffer

Other Notes

Do not used PVDF since free FLAG peptide will bind to the PVDF membrane.
One unit is that amount of enterokinase which results in >95% cleavage of 1 µg of purified FLAG-BAP fusion protein in 18 hours at 37 °C. One FLAG-BAP unit is equal to 10x the activity of a standard trypsinogen unit.

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This Item
E0885E0632SRP3032
Enterokinase from bovine intestine powder

Sigma-Aldrich

E5144

Enterokinase from bovine intestine

Enterokinase from porcine intestine lyophilized powder, ≥100 units/mg protein

Sigma-Aldrich

E0885

Enterokinase from porcine intestine

Enterokinase from porcine intestine ≥0.5 units/mg solid

Sigma-Aldrich

E0632

Enterokinase from porcine intestine

Enterokinase human recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC), suitable for cell culture

Sigma-Aldrich

SRP3032

Enterokinase human

description

powder

description

lyophilized powder, ≥100 units/mg protein

description

≥0.5 units/mg solid

description

recombinant, expressed in CHO cells, ≥90% (SDS-PAGE), ≥90% (HPLC), suitable for cell culture

biological source

bovine

biological source

-

biological source

-

biological source

human

recombinant

expressed in Pichia pastoris

recombinant

-

recombinant

-

recombinant

expressed in CHO cells

form

powder

form

lyophilized powder

form

salt-free, lyophilized powder

form

lyophilized

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

wet ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

42.3 kDa (+/- 6.4 kDa)

mol wt

150 kDa

mol wt

150 kDa

mol wt

108.7 kDa

WGK

WGK 3

Storage Class

11 - Combustible Solids

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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