The volume of the container is 2 mL. Reconstitution of this product should be performed using 0.2 µm filtered 20 mM Tris, pH 7.0, to prepare a stock solution of >= 25 ug/mL. This stock solution may be diluted immediately before use to achieve the final working concentration. Additional filtration is not recommended, as it may result in product loss due to adsorption onto the filter membrane.
F0291
Fibroblast Growth Factor-Basic, human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 25 μg | Available to ship TODAYfromMilliporeSigma Canada Ltd. | $462.00 |
About This Item
Biological source:
human
Recombinant:
expressed in E. coli
Assay:
≥97% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
16.0 kDa
Impurities:
≤1.00 EU/μg
$462.00
Available to ship TODAYDetails
Product Name
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
Quality Level
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg, pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
color
white to faint yellow cast
solubility
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... FGF2 (2247)
General description
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Application
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
Analysis Note
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ~90% (SDS-PAGE and N-terminal analysis) |
| biological source human | biological source human | biological source human | biological source bovine pituitary glands |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| F0291-25UG | 04061838447135 |
| F0291-4X25UG | 04061833611111 |
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What is the volume of the vial and how do I reconstitute the powder?
1 answer-
Helpful?
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Hey! I am using this product to prepare a stem cell media. I know the concentration is 0.025mg but it has two data sheets, one that its soluble in water (0.250ml) and other that says to dissolve in 20mM Tris Base pH 7.0. How many volume should I add?
1 answer-
The solubility of this product is tested in water. It is lyophilized from a solution containing 20 mM Tris and 1M NaCl with 50 ug BSA as a stabilizer. The product data sheet recommends reconstituting in the 20 mM Tris for optimum stability and long term storage.
Helpful?
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