The volume of the container is 2 mL. Reconstitution of this product should be performed using 0.2 µm filtered 20 mM Tris, pH 7.0, to prepare a stock solution of >= 25 ug/mL. This stock solution may be diluted immediately before use to achieve the final working concentration. Additional filtration is not recommended, as it may result in product loss due to adsorption onto the filter membrane.
F0291
Fibroblast Growth Factor-Basic, human
≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Fibroblast Growth Factor-Basic human, FGF2, bFGF, hBFGF
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Product Name
hBFGF, FGF-Basic, recombinant, expressed in E. coli, suitable for cell culture
biological source
human
recombinant
expressed in E. coli
assay
≥97% (SDS-PAGE)
form
lyophilized powder
potency
≤-0.6 ng/mL
mol wt
16.0 kDa
packaging
pkg of 4X25 μg
pkg of 25 μg
storage condition
avoid repeated freeze/thaw cycles
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg
color
white to faint yellow cast
solubility
water: soluble 0.025 mg, clear, colorless to faintly yellow
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
human ... FGF2 (2247)
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This Item | F3685 | F5392 | GF003AF |
|---|---|---|---|
| biological source human | biological source human | biological source bovine pituitary glands | biological source human |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | stem cell: suitable |
| assay ≥97% (SDS-PAGE) | assay ≥97% (SDS-PAGE) | assay ~90% (SDS-PAGE and N-terminal analysis) | assay >95% (SDS-PAGE) |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form - |
Analysis Note
The bioactivity of FGF-Basic was measured in a fluorometric assay using the redox sensitive dye, Resazurin.
Application
General description
Fibroblast growth factor-basic (FGF-Basic) is found in basement membranes and sub-endothelial extracellular matrix. FGF-Basic is induced early in the development of the embryo where it is necessary (but not sufficient) for pluripotency and self-renewal of embryonic stem cells (ESC) and lineage defined stem cells; wherein it functions to support proliferation and inhibit differentiation. Self-renewal of hESC depends upon activation of the Activin/Nodal/Smad2,3 branch and suppression of the BMP/GDF/Smad5 branch of the TGFβ-family cell signaling program. FGF-Basic is a competence factor for Nodal support of pluripotency. Activin-A is necessary and sufficient to maintain stemness. It induces the expression of both Nodal and FGF-Basic in human ES cells.
Physical form
Lyophilized from 0.2 μm filtered solution in 20 mM Tris and 1 M NaCl, pH 7.0, containing 50 μg bovine serum albumin per 1 μg BFGF.
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Description
Pricing
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Lei Xiao et al.
Stem cells (Dayton, Ohio), 24(6), 1476-1486 (2006-02-04)
Human embryonic stem cells (hESCs) self-renew indefinitely while maintaining pluripotency. The molecular mechanism underlying hESCs self-renewal and pluripotency is poorly understood. To identify the signaling pathway molecules that maintain the proliferation of hESCs, we performed a microarray analysis comparing an
R Beklem Bostancioglu et al.
Colloids and surfaces. B, Biointerfaces, 155, 415-428 (2017-05-02)
Accelerated Mesenchymal Stem Cells (MSCs) condensation and robust MSC-matrix and MSC-MSC interactions on nano-surfaces may provide critical factors contributing to such events, likely through the orchestrated signal cascades and cellular events modulated by the extracellular matrix. In this study, human
Chunhui Xu et al.
Stem cells (Dayton, Ohio), 23(3), 315-323 (2005-03-08)
Previous studies have shown that prolonged propagation of undifferentiated human embryonic stem cells (hESCs) requires conditioned medium from mouse embryonic feeders (MEF-CM) as well as matrix components. Because hESCs express growth factor receptors, including those for basic fibroblast growth factor
C Xu et al.
Nature biotechnology, 19(10), 971-974 (2001-10-03)
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least
Brad Davidson et al.
Genes & development, 20(19), 2728-2738 (2006-10-04)
Comprehensive gene networks in Ciona intestinalis embryos provide a foundation for characterizing complex developmental processes, such as the initial phases of chordate heart development. The basic helix-loop-helix regulatory gene Ci-Mesp is required for activation of cardiac transcription factors. Evidence is
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What is the volume of the vial and how do I reconstitute the powder?
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Hey! I am using this product to prepare a stem cell media. I know the concentration is 0.025mg but it has two data sheets, one that its soluble in water (0.250ml) and other that says to dissolve in 20mM Tris Base pH 7.0. How many volume should I add?
1 answer-
The solubility of this product is tested in water. It is lyophilized from a solution containing 20 mM Tris and 1M NaCl with 50 ug BSA as a stabilizer. The product data sheet recommends reconstituting in the 20 mM Tris for optimum stability and long term storage.
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