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Rat Fibronectin

Name: Rat Fibronectin
Brand: SIGMA
Price: 605 USD
Availability: InStock

from rat plasma, powder, suitable for cell culture

Synonym(s):

Fibronectin

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Pack Size	Availability	Price
0.5 mg	Estimated to ship TODAYfromSAINT LOUIS	$605.00
1 mg	Estimated to ship TODAYfromSAINT LOUIS	$1,030.00
2 mg	Estimated to ship onApril 27, 2026fromSAINT LOUIS	$1,950.00

About This Item

UNSPSC Code:

12352202

eCl@ss:

42020141

NACRES:

NA.75

EC Number:

289-149-2

MDL number:

MFCD00131062

Biological source:

rat plasma

Form:

powder

Technique(s):

cell culture | mammalian: suitable

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Properties

Product Name

Fibronectin rat plasma, powder, BioReagent, suitable for cell culture

biological source

rat plasma

Quality Level

200

sterility

sterile

product line

BioReagent

form

powder

packaging

pkg of 0.5 mg, pkg of 1 mg, pkg of 2 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

1‑5 μg/cm²

solubility

H₂O: 1 mg/mL at 37 °C, clear, colorless (some particles)

UniProt accession no.

P04937

binding specificity

Peptide Source: Collagen

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Fn1(25661)

Description

General description

Fibronectin (FN)/FN1 (fibronectin 1) is a multifunctional glycoprotein, produced by hepatic stellate cells (HSCs). It is a constituent of extracellular matrix (ECM). FN1 is found in the cell membrane and cytoplasm. It is also synthesized and liberated by fibroblasts, endothelial cells, macrophages and hepatocytes in vivo.

Application

Fibronectin rat plasma has been used in the induction of differentiation , adhesion to extracellular matrix, neural differentiation [1] and primary neuronal ventral mesencephalon cultures.

Biochem/physiol Actions

Fibronectin (FN)/FN1 (fibronectin 1) plays a major role in cell cycle progression, cell adhesion and proliferation and fibrotic progression. It is essential for for collagen matrix assembly. High expression of FN has a vital role during liver fibrosis. FN1 helps in the support of other matrix proteins.

Preparation Note

This product is lyophilized from 0.05 M Tris buffered saline at pH 7.5. It is soluble at 1 mg/mL in water, and should be allowed to dissolve for at least 30 minutes at 37°C. A small amount of undissolved material may remain, but it will not affect performance. The protein homogeneity is evaluated by immunoelectrophoresis, showing a single arc, is endotoxin tested, and the source material was tested negative for HIV, hepatitis B and hepatitis C.

In coating culture surfaces, fibronectin should be diluted in sterile balanced sterile salt solution and coated with minimal volume. Surface should then be air dried for 45 minutes at room temp and can be stored for 2-4 weeks at 2-8°C.

Analysis Note

A single arc is shown when reacted with anti-fibronectin by IEP (immunoelectrophoresis).

Other Notes

Fibronectin is an adhesive glycoproteins found in both the cell surface and in plasma.

Disclaimer

The reconstituted solution should be stored in working aliquots at -20°C or lower. Vortexing, excessive agitation, or additoinal freezing and thawing of reconstituted fibronectin are not recommended.

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1 of 1

This Item	F2518	F1056	F0162
Sigma-Aldrich F0635 Fibronectin rat plasma	Sigma-Aldrich F2518 Fibronectin human foreskin fibroblasts	Sigma-Aldrich F1056 Fibronectin human plasma	Sigma-Aldrich F0162 Fibronectin Proteolytic Fragment from human plasma
sterility sterile	sterility -	sterility sterile	sterility -
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source rat plasma	biological source human foreskin fibroblasts	biological source human plasma	biological source human plasma
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000502931

0000416688

0000488967

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Protocols

Fibronectin Coating Protocol

Dilute fibronectin for cell attachment, varying per cell type. Coating protocol, products, and FAQs provided.

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Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

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Fibronectin (FN) plays crucial roles in extracellular matrix fibril assembly and cellular interactions.

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Global Trade Item Number

SKU	GTIN
F0635-.5MG	04061833611326
F0635-2MG	04061832951683
F0635-1MG	04061826677681

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