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F1757

Fumarase from porcine heart

ammonium sulfate suspension, ≥300 units/mg protein (biuret)

Synonym(s):

L-Malate hydrolyase, Fumarate hydratase

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500 units
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4.270,00 kr.
2500 units
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16.990,00 kr.
5000 units
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27.010,00 kr.

About This Item

CAS Number:
9032-88-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-887-7
MDL number:
MFCD00131111
EC Number:
4.2.1.2 (BRENDA, IUBMB)
Specific activity:
≥300 units/mg protein (biuret)

4.270,00 kr.

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form

ammonium sulfate suspension

specific activity

≥300 units/mg protein (biuret)

foreign activity

malic dehydrogenase ≤0.1%

storage temp.

2-8°C

General description

Fumarase is mapped to human chromosome 1q43. Fumarase comprises of three domains D1, D2 and D3 and belongs to class II fumarase family.
Fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme found in both the cytoplasm and mitochondria of all eukaryotes.

Application

Fumarase from porcine heart has been used:
  • as a positive control in isocitrate dehydrogenase and α-ketoglutarate dehydrogenase assay
  • for immobilized enzyme based stability assay
  • in fumarate hydratase assays in human lung carcinoma cells (A549)

Fumarase is used as a protein calibration standard in the purification of intact DNA polymerase a/primase from mouse cells.[1]

Biochem/physiol Actions

Fumarase is essential for regulation of cellular metabolism. Fumarase elicits protective function from double-strand breaks (DSBs) induced by infrared radiation. Loss of function mutations in the fumarase gene is implicated in hereditary leiomyomatosis and renal cell cancer (HLRCC)-syndrome.
Fumarase catalyzes the reversible hydration of fumarate to malate. In its mitochondrial form, fumarate is involved in the Krebs Cycle, while the cytosolic form is involved in amino acid metabolism.

Physical form

Suspension in 3.2 M (NH4)2SO4, 0.05 M KH2PO4, pH 7.5, 0.014 M 2-mercaptoethanol

Preparation Note

Isolated by the method of Beeckmans, S., and Kanarek, L., Eur. J. Biochem., 78, 437 (1977). Affinity purified.

Other Notes

One unit will convert 1.0 μmole of L-malate to fumarate per min at pH 7.6 at 25 °C.

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M1567C3260G2751
Fumarase from porcine heart ammonium sulfate suspension, ≥300 units/mg protein (biuret)

Sigma-Aldrich

F1757

Fumarase from porcine heart

Malic Dehydrogenase from porcine heart ≥600 units/mg protein (biuret), ammonium sulfate suspension

Sigma-Aldrich

M1567

Malic Dehydrogenase from porcine heart

Citrate Synthase from porcine heart liquid, ≥100 units/mg protein

Sigma-Aldrich

C3260

Citrate Synthase from porcine heart

Glutamic-Oxalacetic Transaminase from porcine heart Type I, ammonium sulfate suspension, 200-500 units/mg protein

Sigma-Aldrich

G2751

Glutamic-Oxalacetic Transaminase from porcine heart

specific activity

≥300 units/mg protein (biuret)

specific activity

≥600 units/mg protein (biuret)

specific activity

≥100 units/mg protein

specific activity

200-500 units/mg protein

form

ammonium sulfate suspension

form

ammonium sulfate suspension

form

liquid

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

foreign activity

malic dehydrogenase ≤0.1%

foreign activity

Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%

foreign activity

isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%

foreign activity

glutamic-pyruvic transaminase ≤0.03%, lactic dehydrogenase ≤0.01%, malic dehydrogenase ≤0.01%

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

7783-20-2

CAS No.

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