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F2168
Anti-α-Tubulin−FITC antibody, Mouse monoclonal
clone DM1A, purified from hybridoma cell culture
Synonym(s):
Monoclonal Anti-α-Tubulin
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 0.2 mL | Please contact Customer Service for Availability | $794.00 | |
| 0.5 mL | Please contact Customer Service for Availability | $1,310.00 |
About This Item
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
FITC conjugate
Clone:
DM1A, monoclonal
Application:
IF (d)
Citations:
343
biological source
mouse
Quality Level
conjugate
FITC conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
DM1A, monoclonal
form
buffered aqueous solution
mol wt
antigen ~50 kDa
species reactivity
yeast, mouse, amphibian, human, rat, chicken, fungi, bovine
storage condition
protect from light
technique(s)
direct immunofluorescence: 1:50 using cultured BHK cells
isotype
IgG1
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)
General description
α/β-Tubulin, the major building block of microtubules, belongs to the tubulin superfamily of proteins. They are ubiquitously found in all eukaryotic cells. It exists in multiple isotype in many organisms.† It is a heterodimer with intracellular, hollow, cylindrical, filamentous structures. It is located on human chromosome 2q35. α/β-Tubulin and γ-tubulin are members of the tubulin superfamily of proteins. α/β--Tubulin is a heterodimer, which consists of one α-tubulin chain and one β-tubulin chain; each subunit has a molecular weight of 55 kDa and they share considerable homolog. Tubulin is a highly conserved protein with two terminal domains: large N and C terminal.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC.
Monoclonal Anti-α-Tubulin (mouse IgG1 isotype) is derived from the DM1A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Purified chick brain microtubules were used as immunogen. The isotype is determined by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents, Product Number ISO2. The product is Protein A purified Monoclonal Anti-α-Tubulin antibody conjugated to fluorescein isothiocyanate, isomer I. It is purified by gel filtration and contains no detectable free FITC.
Immunogen
Microtubules from chicken embryo brain.
microtubules from chicken embryo brain.
Application
Monoclonal Anti-α-Tubulin antibody is suitable for immunostaining of:
- respiratory epithelium tissue in a study to determine the tubulin expression in the mice cilia
- breast cancer tissue sections to study the effect of LMO4 on the centrosome amplification and mitotic spindle abnormalities
- spindle and chromosomes of oocytes
- detection of tubulin by immunofluorescence and confocal microscopy in lung carcinoma cell.
- immunofluorescent staining of microtubules in human embryos and mitotic spindles from spleen lymphoblast.
Biochem/physiol Actions
The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts.
The antibody is specific for α-tubulin in immunoblotting assays and may be used for localization of α-tubulin in cultured cells or tissue sections. The antibody reacts best with chicken fibroblasts. Anti-α-Tubulin FITC antibody, Mouse monoclonal specifically recognizes an epitope in the carboxy terminal part of α-tubulin. It localizes α-tubulin in human, monkey, bovine, chicken, goat, murine, rat, gerbil, hamster, rat kangaroo, amphibia, sea urchin, trypanosome, yeast, fungi and tobacco.
The N-terminal domain of α−tubulin binds GTP, which is non-exchangeable mechanism, whereas highly acidic C-terminal domain is capable to bind microtubule-associated proteins (MAPs). Both domains are associated with the microtubular self-assembly. Microtubules function as structural and mobile elements in mitosis, intracellular transport, ciliary flagellar motility and generation and maintenance of cell shape. Mutations in tubulin α-4A gene leads to a neurodegenerative disease called amyotrophic lateral sclerosis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin and 15 mM sodium azide
Preparation Note
For continuous use, store at 2-8 °C for a maximum of one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing , or storage in "frost-free" freezers. is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| antibody form purified immunoglobulin | antibody form ascites fluid | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| conjugate FITC conjugate | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| clone DM1A, monoclonal | clone DM1A, monoclonal | clone D66, monoclonal | clone DM1A, monoclonal |
| Gene Information human ... TUBA4A(7277) | Gene Information human ... TUBA4A(7277) | Gene Information human ... TUBB1(81027), TUBB2A(7280), TUBB2C(10383) | Gene Information human ... TUBA4A(7277) |
| species reactivity yeast, mouse, amphibian, human, rat, chicken, fungi, bovine | species reactivity yeast, mouse, amphibian, human, rat, chicken, fungi, bovine | species reactivity human, bovine, chicken, rat, canine, Chlamydomonas reinhardtii, sea urchin (Lytechinus pictus and Paracentrotus lividus), mouse, hamster, Oxyrrhis marina (dinoflagellate), rabbit, monkey | species reactivity yeast, mouse, amphibian, human, rat, chicken, fungi, bovine |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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Articles
Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.
Developmental potential of human oocytes reconstructed by transferring somatic cell nuclei into polyspermic zygote.
Fan y, et al.
Biochemical and Biophysical Research Communications, 382(1), 119-123 (2009)
Yong-Han Kim et al.
Molecular reproduction and development, 86(8), 972-983 (2019-05-29)
Mammalian oocytes lack centrioles but can generate bipolar spindles using several different mechanisms. For example, mouse oocytes have acentriolar microtubule organization centers (MTOCs) that contain many components of the centrosome, and which initiate microtubule polymerization. On the contrary, human oocytes
The Cytoskeleton. An Introductory Survey. Von M. Schliwa. Springer, Wien ? New York 1986. XI, 326 S.: geb. DM 172,? ISBN 3-21 1-81884-7
Herrmann H.
Chem. Zeit., 21(3), 102-103 (1987)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F2168-.5ML | 04061833612798 |
| F2168-.2ML | 04061833612781 |
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What is the immunogen for Monoclonal Anti-α-Tubulin antibody, clone DM1A?
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The immunogen for Monoclonal Anti-α-Tubulin antibody produced in mouse, clone DM1A is microtubules from chicken embryo brain. This antibody recognizes an epitope located at the C-terminal end of the α-tubulin isoform (amino acids 426-430) as noted in the following references:Breitling, F., and Little, M., J. Molec. Biol., 189, 367-370 (1986).Wolff, A., et al., Biol. Cell, 63, 319-326 (1988).Serrano, L., et al., Anal. Biochem., 159, 253-259 (1986).
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