Skip to Content
MilliporeSigma

Key Documents

Skip To

F3040

Monoclonal ANTI-FLAG® M1 antibody

clone M1, purified immunoglobulin, buffered aqueous solution

Promotion Available

Synonym(s):

Anti-ddddk, Anti-dykddddk

Sign In to View Organizational & Contract Pricing.

Spend $500 — Save 5%; Spend $800 — Save 10%; Spend $1,600 — Save 22%*

Sign in to check eligibility. Shop Products

Select a Size

Change View
Size/SKUAvailabilityPrice
0.2 mg
Check Cart for Availability
$669.00
1 mg
Check Cart for Availability
$1,150.00
5 mg
Check Cart for Availability
$2,110.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.32
MDL number:
Conjugate:
unconjugated
Clone:
M1, monoclonal
Application:
WB
Citations:
139

$669.00

Save Up To 22%


Check Cart for Availability

Request a Custom Order
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist


biological source

(Bioreactor)

Quality Segment

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

M1, monoclonal

form

buffered aqueous solution

purified by

using Protein A

species reactivity

all

concentration

2-5 mg/mL

technique(s)

western blot: 10 μg/mL

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

General description

The ANTI-FLAG M1 IgG2b mooclonal antibody binds to proteins with a FLAG marker at the free N-terminus.

Method of purification - Protein A

Immunogen

FLAG; peptide sequence DYKDDDDK

Application

Monoclonal ANTI-FLAG® M1 antibody is suitable for immunoprecipitation, western blotting, or EIA in yeast, animal and E.coli cells.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.


Chromatin immunoprecipitation (1 paper)


Immunofluorescence (1 paper)

Learn more product details in our FLAG® applications portal.

Biochem/physiol Actions

Binds to the FLAG epitope when it is located at the free amino-terminus of a fusion protein. Does not bind to Met-FLAG fusion proteins, so will not recognize unprocessed, cytoplasmically expressed proteins. Binding is Ca2+-dependent; the complex dissociates in the absence of calcium ions.

Physical form

Solution in 10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide (w/v).

Preparation Note

Dilute antibody to 10ug/mL in .05 M TBS, pH 7.4, containing 1 mM CaCl2.

Legal Information

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
F3165F4049A9469
description

clone M1, purified immunoglobulin, buffered aqueous solution

description

clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)

description

clone M2, purified immunoglobulin, buffered aqueous solution

description

clone M2, purified immunoglobulin, buffered aqueous glycerol solution

clone

M1, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

clone

M2, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

alkaline phosphatase conjugate

biological source

(Bioreactor)

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin (Purified IgG1 subclass)

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

all

species reactivity

all

species reactivity

all

species reactivity

all

technique(s)

western blot: 10 μg/mL

technique(s)

western blot: 10 μg/mL (Protein A)

technique(s)

direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone

technique(s)

indirect ELISA: 1:20,000


Still not finding the right product?

Try our Product Selector Tool to narrow your options


flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2



Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library



Questions

Reviews

No rating value

Active Filters