The suitability of the product with formalin-fixed paraffin (FFPE) sections has not been determined . If using FFPE sections with this product, the application must be evaluated by the end user.
F3761
Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Goat Anti-Human Kappa Light Chain (Bound and Free)−Fluorescein isothiocyanate
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 2 mL | Please contact Customer Service for Availability | $771.00 $655.35 |
About This Item
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF (d)
Citations:
8
$655.35
List Price$771.00Save 15%Please contact Customer Service for Availability
biological source
goat
Quality Level
conjugate
FITC conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct immunofluorescence: 1:16
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... IGK@(50802)
General description
Anti-Human κ Light Chain (bound and free) antibody is obtained from goat antiserum. Immunospecific purification removes all goat serum proteins and immunoglobulins that do not bind to the κ light chain (bound and free). Goat anti-κ light chain is conjugated to fluorescein isothiocyanate (FITC). The antibody preparation is specific for human κ light (human IgA, IgG, and IgM) and free κ light chain (Bence Jones κ myeloma protein). The conjugate shows no reaction with bound or free λ light chains.
Anti-Human Kappa Light Chain (Bound and Free)−FITC antibody is specific for human kappa light chain when tested against purified bound kappa light chain (human IgA, IgG, and IgM) and free kappa light chain (Bence Jones Kappa myeloma protein). The conjugate shows no reaction with bound or free lambda light chains.
Mammalian antibodies contain one of two types of light chain, called kappa or lamba. Each chain contains a constant and a variable domain. Mammalian antibodies contain either two kappa or two lambda light chains.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Fluorescein isothiocyanate (FITC) is a fluorescein derivative (fluorochrome) used to tag antibodies, including secondary antibodies, for use in fluorescence-based assays and procedures. FITC excites at 495 nm and emits at 521 nm.
Immunogen
Bence Jones-κ myeloma protein
Application
Anti-Human κ Light Chain (Bound and Free)-FITC antibody may be used for immunofluorescence at a working antibody dilution of 1:16. The antibody was used in flow cytometry analysis in various studies.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Flow cytometry/Cell sorting (1 paper)
Goat polyclonal anti-Human Kappa Light Chain (Bound and Free)−FITC antibody may be used to detect and quantitate the level of kappa light chain containing antibody by fluorescent techniques.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| biological source goat | biological source goat | biological source goat | biological source mouse |
| conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate | conjugate FITC conjugate |
| technique(s) direct immunofluorescence: 1:16 | technique(s) direct immunofluorescence: 1:32 | technique(s) direct immunofluorescence: 1:16 | technique(s) flow cytometry: suitable |
| clone polyclonal | clone polyclonal | clone polyclonal | clone A8B5, monoclonal |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
Still not finding the right product?
Try our Product Selector Tool to narrow your options
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Instructions
Annalee W Nguyen et al.
Methods in molecular biology (Clifton, N.J.), 2070, 397-422 (2019-10-19)
While antibody libraries are traditionally screened in phage, bacterial, or yeast display formats, they are produced in large scale for pharmaceutical and commercial use in mammalian cell lines. The simpler organisms used for screening have significantly different folding and glycosylation
Steffen Dickopf et al.
Biological chemistry (2019-03-14)
A novel bispecific antibody format was applied to generate T cell-engaging antibodies. The TriFab format is a trivalent IgG-shaped entity composed of two Fab arms that bind to antigens on the surface of tumor cells, which are linked via flexible
Frederick W Jacobsen et al.
Journal of immunology (Baltimore, Md. : 1950), 186(1), 341-349 (2010-12-07)
Studies for vaccine and human therapeutic Ab development in cynomolgus monkeys (cynos) are influenced by immune responses, with Ab responses playing a significant role in efficacy and immunogenicity. Understanding the nature of cyno humoral immune responses and characterizing the predominant
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F3761-2ML | 04061837516665 |
-
Can this fluorescine conjugated anti kappa used for direct immunofluorescence on formaline fixed paraffin sections .can I see your positive control
1 answer-
Helpful?
-