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F4125

Ficin from fig tree latex

saline suspension, ≥1.0 units/mg protein (biuret)

Synonym(s):

Debricin, Ficain, higueroxyl delabarre

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Size/SKUAvailabilityPrice
100 units
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$196.00
200 units

Estimated to ship onOctober 23, 2026fromMILWAUKEE

$312.00
1000 units
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$3,880.00

About This Item

CAS Number:
9001-33-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.22.3 (BRENDA, IUBMB)
MDL number:
MFCD00081597
Specific activity:
≥1.0 units/mg protein (biuret)
Biological source:
fig tree (latex)

$196.00

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biological source

fig tree (latex)

Quality Segment

form

saline suspension

specific activity

≥1.0 units/mg protein (biuret)

mol wt

23.8 kDa

storage temp.

2-8°C

General description

Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Application

Ficin from fig tree latex has been used:
  • in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
  • for the digestion of eye tissue sections prior to immunostaining and immunofluorescence

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.

Physical form

Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0

Preparation Note

2× Crystallized

Other Notes

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).

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Ficin from fig tree latex saline suspension, ≥1.0 units/mg protein (biuret)

Sigma-Aldrich

F4125

Ficin from fig tree latex

Ficin from fig tree latex powder, ≥0.1 unit/mg solid

Sigma-Aldrich

F4165

Ficin from fig tree latex

Ficin from fig tree latex lyophilized powder

Sigma-Aldrich

F6008

Ficin from fig tree latex

Sigma-Aldrich

Sigma-Aldrich

56969

Micro particles based on polystyrene, dark red

description

saline suspension, ≥1.0 units/mg protein (biuret)

description

powder, ≥0.1 unit/mg solid

description

lyophilized powder

description

size: 100 μm

biological source

fig tree (latex)

biological source

fig tree (latex)

biological source

fig tree (latex)

biological source

-

specific activity

≥1.0 units/mg protein (biuret)

specific activity

≥0.1 unit/mg solid

specific activity

≥1.0 units/mg protein

specific activity

-

form

saline suspension

form

powder

form

lyophilized powder

form

aqueous suspension

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

100

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000591652
    0000558905
    0000558906
    0000536447
    030M7022V

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