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F4125

Ficin from fig tree latex

saline suspension, ≥1.0 units/mg protein (biuret)

Synonym(s):

Debricin, Ficain, higueroxyl delabarre

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100 units
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$189.00
200 units
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$301.00
1000 units

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$3,740.00

About This Item

CAS Number:
9001-33-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.4.22.3 (BRENDA, IUBMB)
MDL number:
MFCD00081597
Specific activity:
≥1.0 units/mg protein (biuret)
Biological source:
fig tree (latex)

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biological source

fig tree (latex)

Quality Segment

200

form

saline suspension

specific activity

≥1.0 units/mg protein (biuret)

mol wt

23.8 kDa

storage temp.

2-8°C

General description

Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.
Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Application

Ficin from fig tree latex has been used:
  • in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality
  • for the digestion of eye tissue sections prior to immunostaining and immunofluorescence

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.

Physical form

Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0

Preparation Note

2× Crystallized

Other Notes

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).

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description

saline suspension, ≥1.0 units/mg protein (biuret)

description

lyophilized powder

description

powder, ≥0.1 unit/mg solid

description

buffered aqueous suspension, 2× Crystallized, ≥16 units/mg protein

specific activity

≥1.0 units/mg protein (biuret)

specific activity

≥1.0 units/mg protein

specific activity

≥0.1 unit/mg solid

specific activity

≥16 units/mg protein

biological source

fig tree (latex)

biological source

fig tree (latex)

biological source

fig tree (latex)

biological source

-

form

saline suspension

form

lyophilized powder

form

powder

form

buffered aqueous suspension

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.4 kDa

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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