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Ficin from fig tree latex

Name: Ficin from fig tree latex
Brand: SIGMA
Price: 189 USD
Availability: InStock

saline suspension, ≥1.0 units/mg protein (biuret)

Synonym(s):

Debricin, Ficain, higueroxyl delabarre

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Pack Size	Availability	Price
100 units	Available to ship TODAYfromMILWAUKEE	$189.00
200 units	Check Cart for Availability	$301.00
1000 units	Available to ship TODAYfromMILWAUKEE	$3,740.00

About This Item

CAS Number:

9001-33-6

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.22.3 (BRENDA, IUBMB)

MDL number:

MFCD00081597

Specific activity:

≥1.0 units/mg protein (biuret)

Biological source:

fig tree (latex)

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Properties

Quality Segment

200

biological source

fig tree (latex)

form

saline suspension

specific activity

≥1.0 units/mg protein (biuret)

mol wt

23.8 kDa

storage temp.

2-8°C

Description

General description

Extinction Coefficient: E^1% = 21.0 (280 nm)
pI: 9.0

Ficin from fig tree latex exists in multiple isoforms. The isoforms are prone to autolysis at varying temperature and tend to degrade during long term storage. It is a sulfhydryl protease with eight cysteine residues and is stabilized by three disulfide bridges.[1]

Application

Ficin from fig tree latex has been used:

in the electron paramagnetic resonance (EPR) and steady-kinetic measurements for assessing its peroxidase functionality[1]
for the digestion of eye tissue sections prior to immunostaining and immunofluorescence

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported K_m for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)₂ fragments from mouse IgG1.

Physical form

Suspension in 2.0 M NaCl and 0.03 M cysteine, pH 5.0

Preparation Note

2× Crystallized

Other Notes

One unit will produce a ΔA₂₈₀ of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 mL (1 cm light path).

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This Item	F6008	F4165	P3125
Sigma-Aldrich F4125 Ficin from fig tree latex	Sigma-Aldrich F6008 Ficin from fig tree latex	Sigma-Aldrich F4165 Ficin from fig tree latex	Sigma-Aldrich P3125 Papain from papaya latex
specific activity ≥1.0 units/mg protein (biuret)	specific activity ≥1.0 units/mg protein	specific activity ≥0.1 unit/mg solid	specific activity ≥16 units/mg protein
biological source fig tree (latex)	biological source fig tree (latex)	biological source fig tree (latex)	biological source -
form saline suspension	form lyophilized powder	form powder	form buffered aqueous suspension
storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C
mol wt 23.8 kDa	mol wt 23.8 kDa	mol wt 23.8 kDa	mol wt 23.4 kDa
Quality Level 200	Quality Level 300	Quality Level 200	Quality Level 200

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pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Global Trade Item Number

SKU	GTIN
F4125-200UN	04061832700748
F4125-100UN	04061832700724
F4125-1KU	04061832700731

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