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F4165

Ficin from fig tree latex

powder, ≥0.1 unit/mg solid

Synonym(s):

Debricin, Ficain, higueroxyl delabarre

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Size/SKUAvailabilityPrice
1000 units
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$162.00

About This Item

CAS Number:
9001-33-6
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-599-1
MDL number:
MFCD00081597
EC Number:
3.4.22.3 (BRENDA, IUBMB)
Specific activity:
≥0.1 unit/mg solid
Biological source:
fig tree (latex)

$162.00

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biological source

fig tree (latex)

Quality Segment

200

form

powder

specific activity

≥0.1 unit/mg solid

mol wt

23.8 kDa

storage temp.

−20°C

General description

Extinction Coefficient: E1% = 21.0 (280 nm)
pI: 9.0

Biochem/physiol Actions

Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone). Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1.

Other Notes

One unit will produce a ΔA280 of 1.0 per min at pH 7.0 at 37 °C when measuring TCA soluble products from casein in a final volume of 10 ml (1 cm light path).
The enzyme is soluble in 1 M potassium phosphate buffer, pH 7.0 (0.25 mg/ml), yielding a clear solution.

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Ficin from fig tree latex powder, ≥0.1 unit/mg solid

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Sigma-Aldrich

F4125

Ficin from fig tree latex

description

powder, ≥0.1 unit/mg solid

description

lyophilized powder

description

crude powder, 1.5-10 units/mg solid

description

saline suspension, ≥1.0 units/mg protein (biuret)

biological source

fig tree (latex)

biological source

fig tree (latex)

biological source

-

biological source

fig tree (latex)

specific activity

≥0.1 unit/mg solid

specific activity

≥1.0 units/mg protein

specific activity

1.5-10 units/mg solid

specific activity

≥1.0 units/mg protein (biuret)

form

powder

form

lyophilized powder

form

crude powder

form

saline suspension

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.4 kDa

mol wt

23.8 kDa

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

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pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 1

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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