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F9259

Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Mouse IgM antibody

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162659
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF (d)
Citations:
34

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biological source

goat

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

direct immunofluorescence: 1:128

storage temp.

2-8°C

target post-translational modification

unmodified

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
Anti-Mouse IgM (μ-chain specific)-FITC antibody is specific for mouse IgM with mu chain. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM. Goat anti-mouse IgM is then conjugated to Fluorescein Isothiocyanate (FITC), Isomer I.

Immunogen

Purified mouse IgM

Application

Anti-Mouse IgM (μ-chain specific)-FITC antibody may be used for immunofluorescent labeling of mouse spleen cells at a minimum working antibody dilution of 1:128. It was also used for immunohistochemistry of rat heart tissue sections at a working dilution of 1:50. A dilution of 1:100 was used to label human ovarian-adenocarcinoma cells for immunofluorescence.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Binds mouse IgM only; does not bind other mouse Igs.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Mouse IgM (μ-chain specific)–FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

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Anti-Human IgM (μ-chain specific)−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

F5384

Anti-Human IgM (μ-chain specific)−FITC antibody produced in goat

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

direct immunofluorescence: 1:128

technique(s)

direct immunofluorescence: 1:64

technique(s)

direct immunofluorescence: 1:16

technique(s)

direct immunofluorescence: 1:32

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000250999
0000250999
SLCR1913
SLCP7222
SLBX2410

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Global Trade Item Number

SKUGTIN
F9259-.5ML04061837517327
F9259-2ML04061833621462
F9259-1ML04061833621424

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