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F9512

Anti-Human IgG (Fc specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti Human IgG Fitc - Anti-Human IgG (Fc specific)-FITC antibody produced in goat, Anti Human Igg Fitc

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0.5 mL
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$206.00
1 mL
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$340.00
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$518.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162438
Conjugate:
FITC conjugate
Clone:
polyclonal
Application:
IF
Citations:
55

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biological source

goat

Quality Level

200

conjugate

FITC conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

storage condition

protect from light

technique(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Anti-Human IgG (Fc specific)-FITC antibody specifically recognizes Fc fragment of all human IgG subclasses. The conjugate shows no reactivity with human IgG Fab fragment, IgA, IgM and light chains and normal mouse or rat serum proteins. The antibody is purified by immunospecific purification and then conjugated to FITC.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG. The antibody is isolated from goat anti-human IgG antiserum by immunospecific purification, which removes essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the Fc fragment of human IgG.

Immunogen

Purified human IgG Fc fragment

Application

Anti-Human IgG (Fc specific)-FITC antibody produced in goat has been used in:
  • immunofluorescence
  • immunohistochemistry
  • surface labeling experiments

Biochem/physiol Actions

Binds human IgG; does not bind other human Igs.
Provides reduced background with mouse or rat samples
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. It mainly helps in immune defense.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

No cross-reaction with mouse and rat IgG.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human IgG (Fc specific)−FITC antibody produced in goat affinity isolated antibody, buffered aqueous solution

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Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

A9309

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

conjugate

FITC conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

peroxidase conjugate

technique(s)

immunofluorescence: 1:64-1:128 using Hep2 cells

technique(s)

direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25, western blot: 1:50,000-1:100,000 using total cell extract of HeLa cells

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:40,000-1:80,000 using total cell extract of HeLa cells

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

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10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000537745
0000537745
0000526411
0000526412
0000526412

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Global Trade Item Number

SKUGTIN
F9512-2ML04061833621851
F9512-.5ML04061833621813
F9512-1ML04061833621820

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