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G1546

Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal

clone GSN149, purified from hybridoma cell culture

Synonym(s):

Mouse Anti-GFP

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25 μL

Available to ship TODAYfromMILWAUKEE

$208.00
100 μL

Available to ship TODAYfromMILWAUKEE

$626.00
200 μL

Available to ship TODAYfromMILWAUKEE

$812.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
GSN149, monoclonal
Application:
ELISA (i), WB
Citations:
67

$208.00

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biological source

mouse

Quality Segment

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GSN149, monoclonal

form

buffered aqueous solution

packaging

antibody small pack of 25 μL

technique(s)

indirect ELISA: suitable, western blot: 1-2 μg/mL using extracts of cells expressing GFP fusion proteins

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Monoclonal Anti-Green Fluorescent Protein (GFP) (mouse IgG1 isotype) is derived from the hybridoma GSN149 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of the Green fluorescent protein from jellyfish Aequorea victoria. GFP is a 27 kDa protein (238 amino acids) that can produce a strong green fluorescence without the need for a substrate. It absorbs light maximally at 395 nm and emits a bright green fluorescence with a peak at 509 nm. The GFP chromophore is formed through cyclization and oxidation of an internal tripeptide motif (Ser65, Gly69 and Tyr66).
The antibody reacts specifically with GFP-fusion proteins.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Monoclonal Anti-Green Fluorescent Protein (GFP) antibody produced in mouse was used in surface plasmon resonance (SPR) gold chip analysis to study the cysteine- and domain-mediated immobilization of enhanced GFP model protein.

Biochem/physiol Actions

GFP is implicated in protein detection and localization in living cells, as well as gene expression monitoring in prokaryotes and eukaryotes. It detects protein-protein interactions in living cells.
Green fluorescent protein, produced in the jellyfish, Aequorea victoria, acts as secondary fluorescent protein in an energy transfer reaction to produce green light. It ability to produce a highly visible, internal fluorophore is widely applicable as marker for gene expression and to produce chimeric proteins to understand protein biochemistry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal clone GSN149, purified from hybridoma cell culture

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Anti-Green Fluorescent Protein (GFP) antibody, Mouse monoclonal

Anti-GFP, N-terminal antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sigma-Aldrich

G1544

Anti-GFP, N-terminal antibody produced in rabbit

description

clone GSN149, purified from hybridoma cell culture

description

clone GFP-20, ascites fluid

description

clone GFP-20, purified from hybridoma cell culture

description

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

clone

GSN149, monoclonal

clone

GFP-20, monoclonal

clone

GFP-20, monoclonal

clone

polyclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified antibody

antibody form

affinity isolated antibody

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

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