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G2500

Gelatin from porcine skin

gel strength 300, Type A

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여러분께/SKU재고 정보가격 (VAT 별도)
100 g

오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea

₩110,838
500 g

오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea

₩217,210
1 kg

오늘 배송 가능재고 수량 조회Seoul Warehouse South Korea

₩396,358

제품정보 (DICE 배송 시 비용 별도)

CAS 번호:
9000-70-8
MDL number:
MFCD00214058
EC Number:
232-554-6
NACRES:
NA.61
UNSPSC Code:
12352202
Form:
powder
Assay:
70-90% (biuret)
Biological source:
Porcine skin

₩110,838

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biological source

Porcine skin

Quality Segment

200

type

Type A

assay

70-90% (biuret)

form

powder

technique(s)

cell culture | mammalian: suitable

gel strength

~300 g Bloom

solubility

H2O: soluble 50 mg/mL

General description

Gelatin is a hydrocolloid. Bovine or porcine bone and hide are its commercial sources.[1] It is a collagen derivative and comprises protein as a major component with minerals and water.[2]

Application

Gelatin from porcine skin has been used:
  • for coating glass-bottomed plates for culturing A375 cells and in gelatin degradation assay[1]
  • for coating glass slides in the histological studies with brain sections[3]
  • in the synthesis of gelatin methacrylate (GelMA) prepolymer for encapsulating cells on to 3D printed hydrogels[4]
  • to prepare thiol-functionalized gelatin (GelSH) hydrogel for 3D projection bioprinting[5]

Gelatin from porcine skin is recommended for use as a cell culture substratum at 1-5 μg/cm2 or 0.5-50 μg/mL. The optimal concentration does depend on cell type as well as the application and research objectives.

Biochem/physiol Actions

Gelatin polymer is an alternative biomaterial to collagen for cell and tissue culture.[2] It is a much sorted gelling agent useful as a thickener and food ingredient. Gelatin is also used as a foaming and binding agent and as well as in the pharmaceutical industry.[1] It is biodegradable and biocompatible with biomedical applications especially in biomaterials for cardiac and vascular tissue regeneration.[2]

Preparation Note

This product is derived from porcine skin. Gelatin is soluble in hot than in cold water. It is practically insoluble in most organic solvents such as alcohol, chloroform, carbon disulfide, carbon tetrachloride, ether, benzene, acetone, and oils. The Bloom number, determined by the Bloom gelometer, is an indication of the strength of a gel formed from a solution of the known concentration. The Bloom number is proportional to the average molecular mass. Bloom numbers of porcine skin Gelatin vary from 90 to 300 g. This product has a gell strength of 300 and is soluble in water at 50 mg/mL.

Other Notes

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Gelatin is a heterogeneous mixture of water-soluble proteins of high average molecular masses, present in collagen. Proteins are extracted by boiling the relevant skin, tendons, ligaments, bones, etc. in water. Type A gelatin is derived from acid-cured tissue. Type B is derived from lime-cured tissue.

Disclaimer

Dry gelatin, when stored in airtight containers at room temperature, will remain unchanged for many years. When heated at 100°C in the presence of air, it swells becomes soft and disintegrates to a carbonaceous mass with evolution of pyridine bases and ammonia.

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이 품목
48722G9391G9136
Gelatin from porcine skin gel strength 300, Type A

Sigma-Aldrich

G2500

Gelatin from porcine skin

Gelatin from porcine skin medium gel strength, suitable for microbiology

Millipore

48722

Gelatin from porcine skin

Gelatin from bovine skin Type B, powder, BioReagent, suitable for cell culture

Sigma-Aldrich

G9391

Gelatin from bovine skin

Gelatin from porcine skin Type A, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Sigma-Aldrich

G9136

Gelatin from porcine skin

assay

70-90% (biuret)

assay

-

assay

-

assay

-

biological source

Porcine skin

biological source

Porcine

biological source

bovine skin

biological source

Porcine skin

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

powder

form

powder

form

powder

form

lyophilized powder

solubility

H2O: soluble 50 mg/mL

solubility

H2O: 67 mg/mL at 50 °C, slightly hazy, slightly yellow

solubility

H2O: soluble 50 mg/mL, hazy to strongly hazy, faintly yellow to yellow

solubility

soluble 20 mg/mL, clear, colorless (in warm tissue culture medium)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

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저장 등급

11 - Combustible Solids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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시험 성적서(COA)

Lot/Batch Number

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  1. What is the molecular weight of this Gelatin?

    What is the molecular weight of this Gelatin?

    1 답변

    1. The molecular weight of this product has not been evaluated, However, the molecular weight is closely related to the Bloom Number. The estimated ranges are as follows:
      Bloom Number Average Molecular Mass (Da)
      50−125 (Low Bloom) 20,000−25,000
      175−225 (Medium Bloom) 40,000−50,000
      225−325 (High Bloom) 50,000−100,000

      Please see the link below to review the general datasheet for Gelatin:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/333/625/g9382pis.pdf

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  2. what is the amino group content of the product Gelatine from porcine skin G2500?

    1 답변

    1. The amino group content of Gelatin is not determined in-house.

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  3. What's the molecular weight of this gelatin

    1 답변

    1. The average molecular weight is between 50,000−100,000 Da.

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