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G3153

β-Galactosidase from Escherichia coli

lyophilized powder, ≥500 units/mg protein

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase

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Size/SKUAvailabilityPrice
5 mg

Available to ship TODAYfromMILWAUKEE

$516.00

About This Item

CAS Number:
9031-11-2
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
MFCD00155434
EC Number:
3.2.1.23 (BRENDA, IUBMB)
Specific activity:
≥500 units/mg protein
Recombinant:
expressed in E. coli

$516.00

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recombinant

expressed in E. coli

Quality Segment

200

form

lyophilized powder

specific activity

≥500 units/mg protein

mol wt

465 kDa

storage temp.

−20°C

General description

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Application

β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.
β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).

Biochem/physiol Actions

β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Packaging

Package size based on protein content

Physical form

Stabilized with phosphate buffer and sucrose

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

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G5160

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description

lyophilized powder, ≥500 units/mg protein

description

Grade VI, lyophilized powder, ≥250 units/mg protein

description

Grade VIII, lyophilized powder, ≥500 units/mg protein

description

≥8.0 units/mg solid

specific activity

≥500 units/mg protein

specific activity

≥250 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥8.0 units/mg solid

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

solid

mol wt

465 kDa

mol wt

465 kDa

mol wt

465 kDa

mol wt

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

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