A search of the website does not show a density for product G4155. For products supplied in small quantities, it is not common for the density to be measured. However, for solutions available in larger quantities, density information is often provided. Although the density for G4155 is not stated, there is some useful information available from the website. The physical form of the product is a suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, and 0.5 mM mercaptoethanol at pH 7.4.
G4155
β-Galactosidase from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Synonyme(s) :
β-
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| À vous/Référence | Disponibilité | Prix |
|---|---|---|
1000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 112,00 € |
3000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 289,00 € |
5000 units | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 413,00 € |
A propos de cet article
Numéro CAS:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥500 units/mg protein (biuret)
112,00 €
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aqueous glycerol suspension
Quality Segment
specific activity
≥500 units/mg protein (biuret)
mol wt
465 kDa
storage temp.
−20°C
General description
Poids moléculaire du tétramère : 465 kDa (chaque sous-unité ayant 116,3 kDa)
Application
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Biochem/physiol Actions
βLa β-galactosidase clive le lactose en ses constituants monosaccharides, le glucose et le galactose. Elle catalyse également la transglycosylation du glucose en allolactose, inducteur de la β-galactosidase, dans une boucle de rétroaction.
Physical form
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Other Notes
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D -galactoside to o-nitrophenol and D -galactose per min at pH 7.3 at 37 °C.
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Cet article | |||
|---|---|---|---|
| description aqueous glycerol suspension, ≥500 units/mg protein (biuret) | description Grade VI, lyophilized powder, ≥250 units/mg protein | description ≥8.0 units/mg solid | description ≥2600 units/g |
| specific activity ≥500 units/mg protein (biuret) | specific activity ≥250 units/mg protein | specific activity ≥8.0 units/mg solid | specific activity ≥2600 units/g |
| form aqueous glycerol suspension | form lyophilized powder | form solid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 465 kDa | mol wt 465 kDa | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
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Classe de stockage
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Could you please advise if the density of the aqueous suspension of beta galactosidase from Escherichia coli (G4155) can be assumed as 1 uL of solution is equal to 1 mg, given that the SDS and provided enzyme assay do not contain this information?
1 answer-
Helpful?
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We recently purchased this enzyme. Information given regarding enzyme volume is only 1000 units. I want to know what would be units per uL. How much is the liquid volume in the given product to calculate the enzyme concentration? Provide proper informatio
1 answer-
Units/ml can be calculated using the numbers reported in batch specific CofA. Below is an example.
CofA of G4155, lot 0000303635
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/G4155-3KU0000303635.pdf
1.5 mgP/ml X 766 units/mg Protein = 1149 units/mlHelpful?
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