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G4505

Guanidine hydrochloride

≥99% (titration), organic base and chaeotropic agent

1 of 1 reviewers took part in a promotion

Synonym(s):

Guanidium Chloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride

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25 g

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$52.90
100 g

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$98.60
500 g

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$267.00
1 kg

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$504.00
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$927.00
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About This Item

Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
UNSPSC Code:
12352107
NACRES:
NA.28
PubChem Substance ID:
EC Number:
200-002-3
Beilstein/REAXYS Number:
3591990
MDL number:

$52.90


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biological source

synthetic (organic)

Quality Segment

assay

≥99% (titration)

form

crystalline powder

technique(s)

RNA extraction: suitable

impurities

≤0.3% water (Karl Fischer)

color

white

pH

(25 °C, 4.6 - 6 at 573 g/L)

mp

180-185 °C (lit.)

solubility

H2O: 6 M

density

1.3 g/cm3 (lit.)

absorption

≤0.10 at 260 nm in H2O at 6 M

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

General description

Guanidine Hydrochloride is a potent protein denaturant extensively utilized in the purification of proteins and nucleic acids. Widely recognized for its versatility, it plays a pivotal role in total RNA extraction experiments, dissolving proteins and disrupting cell structures while inactivating RNA enzymes. Beyond denaturation, Guanidine Hydrochloride is instrumental in renaturing proteins, effectively restoring their active states. It also finds widespread applications in sequencing, molecular weight determinations, and acts as a solubilizing agent for electrophoresis. In summary, Guanidine Hydrochloride stands as a crucial tool in biochemical research, offering indispensable capabilities in the purification, denaturation, and renaturation of proteins and nucleic acids across diverse laboratory applications.

Application

Guanidine hydrochloride (GdnHCl) has been used:
  • to perform absolute quantitation of amyloid-beta (Aβ) isoforms in plasma
  • in protein carbonylation assay
  • for the quantification of glycosaminoglycans (GAG)
  • for denaturation of cow′s milk allergens
  • as a control in antiviral screening tests with coxsackievirus B3 (CVB3)
  • in RNA isolation to dissociate nucleoproteins and inhibit RNase
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Biochem/physiol Actions

Guanidine hydrochloride (GdnHCl) is hydroscopic and a small molecule. It is a potent denaturant of enzymes, which results in conformational changes in the enzyme, leading to complete loss of enzyme activity. GdnHCl is involved in blocking the activity of heat shock protein 104 (Hsp104) adenosine triphosphatase (ATPase) in vivo. It is involved in denaturing alkaline phosphatase (ALPase) in Haliotis diversicolor. GdnHCl leads to the deactivation of enzymes like papain and aminoacylase. GdnHCl, at higher concentrations, is effective in virus inactivation, such as herpes simplex virus 1 (HSV-1).

Features and Benefits

  • Suitable for Biochemical and Cell Biology research
  • Ideal for RNA extraction
  • High purity product for research applications

Packaging

G4505-1KT:
Each kit contains 3 x 100G samples, each sample from a uniquely manufactured lot.

Preparation Note

In order to make an 8M solution in water, one must heat the solution to 35 °C for approximately 30 minutes.
The maximum solubility of guanidine hydrochloride in water at room temperature is approximately 6M.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

May agglomerate upon storage. The quality of the product does not appear to be affected and solutions prepared from the free-flowing and lumpy guanidine hydrochloride appear identical. For free-flowing material, please see our Redi-Dri free-flowing product number RDD001.

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Show Differences

1 of 1

This Item
5094050937G9284
description

≥99% (titration), organic base and chaeotropic agent

description

≥99.0% (AT)

description

BioUltra, ~8 M in H2O

description

Colorless liquid, 7.8 - 8.3 M, pH- 4.5 - 5.5

technique(s)

RNA extraction: suitable

technique(s)

-

technique(s)

-

technique(s)

-

assay

≥99% (titration)

assay

≥99.0% (AT)

assay

-

assay

-

biological source

synthetic (organic)

biological source

-

biological source

-

biological source

-

solubility

H2O: 6 M

solubility

H2O: 6 M at 20 °C, clear, colorless

solubility

-

solubility

-

Quality Level

400

Quality Level

200

Quality Level

100

Quality Level

200

form

crystalline powder

form

solid

form

liquid

form

liquid


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Pictograms

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Signal Word

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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