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G6008

β-Galactosidase from Escherichia coli

Grade VI, lyophilized powder, ≥250 units/mg protein

1 of 1 reviewers took part in a promotion

Synonym(s):

β-D-Galactoside galactohydrolase, Lactase

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Size/SKUAvailabilityPrice
1000 units

Available to ship TODAYfromMILWAUKEE

$111.00
5000 units

Available to ship TODAYfromMILWAUKEE

$324.00
10000 units

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$584.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-864-1
MDL number:
EC Number:
Specific activity:
≥250 units/mg protein
Biological source:
Escherichia coli

$111.00


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biological source

Escherichia coli

Quality Segment

type

Grade VI

form

lyophilized powder

specific activity

≥250 units/mg protein

mol wt

465 kDa

does not contain

BSA as extender

composition

Protein, ≥50% biuret

shipped in

wet ice

storage temp.

−20°C

General description

β-Galactosidase is a tetramer consisting of four equal subunits of 135,000 Da each. It is a sulfhydryl containing enzyme, with 19 cysteine residues per subunit.
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Application

β-Galactosidase is conjugated to an antibody that specifically recognizes a target molecule (enzyme immunoassay or EIA). β-Galactosidase is also used as a reporter enzyme to monitor the level of gene expression of a promoter.

Biochem/physiol Actions

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

Physical form

Partially purified; contains Tris buffer salts, magnesium chloride, DL-dithiothreitol and 2-mercaptoethanol

Other Notes

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

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G5635G315310105031001
description

Grade VI, lyophilized powder, ≥250 units/mg protein

description

Grade VIII, lyophilized powder, ≥500 units/mg protein

description

lyophilized powder, ≥500 units/mg protein

description

from E. coli overproducer

specific activity

≥250 units/mg protein

specific activity

≥500 units/mg protein

specific activity

≥500 units/mg protein

specific activity

-

biological source

Escherichia coli

biological source

-

biological source

-

biological source

Escherichia coli

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

suspension

mol wt

465 kDa

mol wt

465 kDa

mol wt

465 kDa

mol wt

540 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

wet ice


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Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1



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Questions

Reviews

1 of 1 reviewers took part in a promotion

Active Filters

    • Review 1
    • Votes 7
    1 out of 5 stars.

    Heterogeneous

    We were trying to use this as a protein standard and found it to be a heterogeneous mess. Not sure what all is in here, but its a lot more than betagalactosidase

    Helpful?