The immunogen for this antibody has not been sequenced, but sample sequences can be found on the NCBI website. The sequence homology between the human protein sequence and the mouse protein sequence is 92.29%. Typically, for polyclonal antibodies, an 85% or greater homology ensures binding, indicating that this product should work for mice.
G9269
Anti-Glial Fibrillary Acidic Protein Antibody
rabbit polyclonal
Synonym(s):
GFAP Antibody Sigma - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, Gfap Antibody Sigma, Anti-GFAP
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| Size/SKU | Availability | Price |
|---|---|---|
25 μL | Available to ship TODAYfromMILWAUKEE | $194.00 |
0.2 mL | Available to ship TODAYfromMILWAUKEE | $651.00 |
1 mL | Available to ship TODAYfromMILWAUKEE | $1,300.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, IHC (p), WB
Citations:
409
Product Name
Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
rat, human
packaging
antibody small pack of 25 μL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using brain sections, microarray: suitable, western blot: 1:500 using brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GFAP(2670)
rat ... Gfap(24387)
General description
The GFAP (glial fibrillary acidic protein) gene encodes an intermediate protein filament-III (50kDa). It is localized particularly to the astrocytes and non-myelinating Schwann cells of the central and peripheral nervous system, respectively.
Immunogen
GFAP from human brain
Application
Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit is suitable for:
- immunohistochemical staining at a working dilution of 1:400 using rat brain sections
- immunohistochemical staining of human brain tumor tissue specimens to identify the cells expressing AQP4 (Aquaporin-4)
- immunofluorescence using brain section from mice
Biochem/physiol Actions
Glial fibrillary acidic protein may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. GFAP is responsible for maintaining the structure of glial cells. It also offers mechanical strength and support to the surrounding neurons and blood brain barrier. Defects in this gene causes Alexander disease, indicated by Rosenthal fibers deposition. It is a rare disorder of astrocytes in the central nervous system. Increased GFAP expression indicates astrogliosis, a sign of neurodegeneration and neuroinflammation.
The product reacts specifically with GFAP and labels astrocytes, glial cells and glial cell derived tumors in immunohistochemical staining. The antibody reacts with glial specific antigen in alcohol or formalin-fixed, paraffin-embedded human or animal tissue sections.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide
Preparation Note
Store at 2-8 °C up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description IgG fraction of antiserum, buffered aqueous solution | description clone G-A-5, purified from hybridoma cell culture | description clone G-A-5, ascites fluid | description Chemicon®, from chicken |
| antibody form IgG fraction of antiserum | antibody form purified immunoglobulin, purified from hybridoma cell culture | antibody form ascites fluid | antibody form affinity isolated antibody |
| biological source rabbit | biological source mouse | biological source mouse | biological source chicken |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate - |
| technique(s) western blot: 1:500 using brain extract, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using brain sections, microarray: suitable | technique(s) immunohistochemistry: suitable, immunocytochemistry: suitable, indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum., western blot: suitable | technique(s) indirect immunofluorescence: suitable, microarray: suitable, immunoblotting: suitable, immunocytochemistry: suitable, immunohistochemistry: 1:400 using rat brain sections (alcohol-fixed) | technique(s) western blot: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunocytochemistry: suitable |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
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