The product data sheet only includes information for indirect immunofluorescence (IF). However, a cited publication in the American Journal of Physiology - Heart and Circulatory Physiology, dated March 1, 2015, suggest a protocol for immunohistochemistry (IHC).
G3893
Anti-Glial Fibrillary Acidic Protein Antibody
mouse monoclonal, G-A-5
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse
Synonym(s):
Anti Gfap, Anti-Gfap, GFAP Antibody - Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, Gfap Antibody, Glial Fibrillary Acidic Protein Antibody, Anti-GFAP
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100 μL
$452.00
0.2 ML
$661.00
$452.00
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
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Product Name
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse, clone G-A-5, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
G-A-5, monoclonal
species reactivity
rat, human, pig
technique(s)
immunoblotting: suitable
immunocytochemistry: suitable
immunohistochemistry: 1:400 using rat brain sections (alcohol-fixed)
indirect immunofluorescence: suitable
microarray: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... GFAP(2670)
rat ... Gfap(24387)
1 of 4
This Item | G6171 | G9264 | SAB2500462 |
|---|---|---|---|
| biological source mouse | biological source mouse | biological source mouse | biological source goat |
| antibody form ascites fluid | antibody form purified from hybridoma cell culture, purified immunoglobulin | antibody form ascites fluid | antibody form affinity isolated antibody |
| species reactivity rat, human, pig | species reactivity rat, human, pig | species reactivity hamster, human, feline, rat, chicken, snake, mouse | species reactivity rat |
| Gene Information human ... GFAP(2670) | Gene Information human ... GFAP(2670) | Gene Information human ... GAP43(2596) | Gene Information human ... GFAP(2670) |
| clone G-A-5, monoclonal | clone G-A-5, monoclonal | clone GAP-7B10, monoclonal | clone polyclonal |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
General description
Monoclonal anti-glial fibrillary acidic protein (GFAP) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP is the intermediate filament expressed in astrocytes. The gene encoding it is localized on human chromosome 17q21.31.
The isotype is determined using ImmunoTypeTM Kit (Product Code ISO-1) and by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Product Code ISO-2).
Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. GFAP (molecular weight of 50 kDa) is the cell specific IF protein in astrocytes.
The isotype is determined using ImmunoTypeTM Kit (Product Code ISO-1) and by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Product Code ISO-2).
Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. GFAP (molecular weight of 50 kDa) is the cell specific IF protein in astrocytes.
Immunogen
GFAP from pig spinal cord
Application
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) antibody produced in mouse is suitable for immunohistochemistry at a working dilution of 1:400 using rat brain sections (alcohol-fixed) and microarray analysis. It may be used for immunocytochemical localization of GFAP in human, pig, and rat tissues. It is also suitable for localization of GFAP using immunoblot assays. In indirect immunofluorescent labeling on alcohol-fixed or frozen sections, this antibody stains astrocytes and Bergmann glia cells, gliomas, and other glial cell derived tumors.
The antibody was used as a primary antibody in immunocytochemistry analysis:
The antibody was used as a primary antibody in immunocytochemistry analysis:
- of primary cerebral microvascular EC cultures to study the effect of microglia on the BBB (blood-brain barrier) and its primary constituents
- to study the wound healing effects of matrix metalloproteinase-2 that promote recovery after spinal cord injury
- to study the negative regulation of embryonic neural progenitor cell proliferation by Toll-like receptor 3
Biochem/physiol Actions
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, Bergmann glia cells and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.
The gene GFAP encodes an intermediate filament protein (50kDa) of mature astrocytes, which may be used as a marker for distinguishing astrocytes from other glial cells during development of the central nervous system. Defects in this gene causes Alexander disease. It is a rare disorder of astrocytes in the CNS.
Physical form
Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) is provided as ascites fluid containing 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Molecular Components of the Neuronal Cytoskeleton
Siegel G J, et al.
Basic Neurochemistry: Molecular, Cellular and Medical Aspects. (1999)
Characterization of a panel of monoclonal antibodies recognizing specific epitopes on GFAP.
Lin N H, et al.
PLoS ONE, 12(7), e0180694-e0180694 (2017)
Potentiation of Astrogliogenesis by STAT3-Mediated Activation of Bone Morphogenetic Protein-Smad Signaling in Neural Stem Cells? ?
Shinji Fukuda, et al.
Journal of Virology, 27(13) (2007)
Fernando Ezquer et al.
Addiction biology, 24(5), 994-1007 (2018-09-22)
Chronic ethanol consumption leads to brain oxidative stress and neuroinflammation, conditions known to potentiate and perpetuate each other. Several studies have shown that neuroinflammation results in increases in chronic ethanol consumption. Recent reports showed that the intra-cerebroventricular administration of mesenchymal
Tomokatsu Yoshida et al.
Journal of human genetics, 58(9), 635-638 (2013-08-02)
Alexander disease (AxD) is a rare neurodegenerative disorder. Most patients with AxD have a de novo dominant missense mutation in the glial fibrillary acidic protein (GFAP) gene. Patients with late-onset AxD exhibit a more variable onset and severity than patients
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