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H1643

Anti-Hamster IgG (whole molecule) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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1 mL
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$252.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162371
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i)
Citations:
6

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biological source

rabbit

Quality Segment

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

technique(s)

indirect ELISA: 1:100,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG is present in large quantities in the human serum. IgG is composed of glycoproteins, out of which it is 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds. The heavy chains consist of a N-terminal variable domain (VH) and three constant domains (CH1, CH2, CH3). A hinge region exists between the CH1 and CH2 region. The light chains have one N-terminal variable domain (VL) and one constant domain (CL). The heavy and the light chains are linked at VH and CH1 domain to form the Fab arm (Fragment antigen binding). The antigen binds to the V regions of the antibody.

Application

Anti-Hamster IgG (whole molecule) antibody produced in rabbit is suitable for use in Immunoelectron Microscopy and FACS.

Biochem/physiol Actions

IgG antibody subtype is the most abundant serum immunoglobulin of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
IgG, a monoclonal antibody can be cleaved at the hinge region by nonspecific proteases like papain and pepsin. This can result in univalent Fab (Fragment antigen binding) fragments or bivalent F(ab′)2 fragments. These two enzymes have a broad substrate specificity resulting in heterogenous fragments.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Hamster IgG (whole molecule) antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

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Anti-Mouse IgG (whole molecule) antibody produced in goat whole antiserum

Sigma-Aldrich

M5899

Anti-Mouse IgG (whole molecule) antibody produced in goat

description

affinity isolated antibody, buffered aqueous solution

description

IgG fraction of antiserum, buffered aqueous solution

description

affinity isolated antibody, buffered aqueous solution

description

whole antiserum

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

whole antiserum

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

technique(s)

indirect ELISA: 1:100,000

technique(s)

indirect ELISA: 1:80,000, quantitative precipitin assay: 2.0 mg/mL

technique(s)

indirect ELISA: 1:20,000

technique(s)

indirect ELISA: 1:70,000, quantitative precipitin assay: 3.2-4.8 mg/mL

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

goat

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

-

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Storage Class

12 - Non Combustible Liquids

WGK

nwg

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