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H1759

(+)-Biotin N-hydroxysuccinimide ester

≥98% (HPLC), powder

Synonym(s):

(+)-Biotin N-succinimidyl ester, d-Biotin NHS ester, BNHS, Biotinyl-N-hydroxy-succinimide, N-Hydroxysuccinimidobiotin, N-Succinimidyl D-biotinate, NHS-Biotin

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Size/SKUAvailabilityPrice
5 mg

Available to ship TODAYfromMILWAUKEE

$29.50
25 mg

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$78.90
100 mg

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$215.00
250 mg

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$379.00
1 g

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$1,110.00

About This Item

Empirical Formula (Hill Notation):
C14H19N3O5S
CAS Number:
Molecular Weight:
341.38
UNSPSC Code:
12352203
NACRES:
NA.46
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
4720781

$29.50


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Quality Segment

assay

≥98% (HPLC)

form

powder

reaction suitability

reaction type: Biotinylations

color

white to off-white

mp

212 °C

solubility

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF), DMSO: soluble

storage temp.

−20°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(=O)ON3C(=O)CCC3=O)[C@@]1([H])NC(=O)N2

InChI

1S/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

InChI key

YMXHPSHLTSZXKH-RVBZMBCESA-N

Application

(+) Biotin NHS ester or (+)-Biotin N-hydroxysuccinimide ester has been used:


  • to biotinylate Hyb 246-4 for surfactant protein D enzyme-linked immunosorbent assay (SP-D ELISA)
  • to biotinylate proteins to form preformed complexes
  • to label and modify monoclonal anti-microfibrillar-associated protein 4 (MFAP4) (HG-HYB 7-18) to bind to alphaLISA streptavidin-coated donor beads

Biochem/physiol Actions

The N-hydroxysuccinimide ester (NHS)-biotin modification helps in the biotinylation of the target lysine residue at its ε-amino group and identifies the surface lysine residues. It is used as a tool to identify the interaction of proteins with several biological molecules, like protein-polysaccharide interactions, protein-protein interactions, protein-nucleic acid interactions, and protein-ligand interactions. NHS-biotin may be used in the biotinylation of proteins and peptides. It is typically coupled to primary amine in the pH range of 6.5-8.5.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
203112B2643B3295
description

≥98% (HPLC), powder

description

-

description

≥98% (TLC), powder

description

≥95% (TLC), powder

assay

≥98% (HPLC)

assay

≥90% (NMR)

assay

≥98% (TLC)

assay

≥95% (TLC)

form

powder

form

solid

form

powder

form

powder

solubility

DMF: ≤50 mg/mL (Stable for at least one month in dry DMF), DMSO: soluble

solubility

DMSO: 100 mg/mL

solubility

DMF: 50 mg/mL (Stable up to 2 months in dry DMF.), H2O: ≤2 mg/mL (with sonication)

solubility

DMF: 25 mg/mL

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

color

white to off-white

color

white to off-white

color

-

color

-

mp

212 °C

mp

-

mp

-

mp

-


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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