This product may be reconstituted at 1 mg/mL in Tris-HCl, pH 7.5, containing 50 mM NaCl, 4 mM CaCl2, and 0.01% BSA. The solutio is stable for at least one week at -20 °C or 3 months at 70 °C. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/215/379/h2519pis-ms.pdf
H2519
Heparinase I from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin lyase I, Heparinase
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| Size/SKU | Availability | Price |
|---|---|---|
50 units | Available to ship TODAYfromMILWAUKEE | $410.00 |
100 units | Available to ship TODAYfromMILWAUKEE | $737.00 |
250 units | Available to ship TODAYfromMILWAUKEE | $1,570.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg protein (enzyme + BSA)
$410.00
Available to ship TODAYDetails
conjugate
conjugate (Glucosaminoglycan)
Quality Segment
form
lyophilized solid
specific activity
≥200 units/mg protein (enzyme + BSA)
mol wt
42.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
Biochem/physiol Actions
Heparinase I from Flavobacterium heparinum acts by cleaving the α-glycoside linkage at AT III binding site in heparin. This results in small oligosaccharide fragments . Heparinase I neutralizes the protein′s action of anticoagulation. Heparinase I possesses a half-life of 15-18 minutes.
Other Notes
One unit (Sigma unit) will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| description Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA) | description Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA) | description lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA) | description Type II-S, lyophilized powder, Protein ≥80 %, ≥2,000 units/mg protein |
| specific activity ≥200 units/mg protein (enzyme + BSA) | specific activity ≥30 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein | specific activity ≥2,000 units/mg protein |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) diagnostic assay manufacturing |
| form lyophilized solid | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 42.8 kDa | mol wt 70.8 kDa | mol wt - | mol wt ~31 kDa |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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