The solubility of this material is tested at 50 mg/mL in a 1:1 mixture of chloroform and ethanol. The maximum solubility in ethanol has not been determined. However, various literature sources indicate that Hydrocortisone is soluble in absolute ethanol up to 15 mg/mL. Preparing a 10 mM stock solutions of 3.625 mg/mL is expected to be suitable. This stock solution may then be diluted to a working concentration of 1 µM in culture medium.
H4001
Hydrocortisone
synthetic (organic), ≥98% (HPLC), anti-inflammatory agent, powder
Synonym(s):
11β,17α,21-Trihydroxypregn-4-ene-3,20-dione, 17-Hydroxycorticosterone, 4-Pregnene-11β,17α,21-triol-3,20-dione, Cortisol, Kendall’s compound F, Reichstein’s substance M
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| Size/SKU | Availability | Price |
|---|---|---|
1 g | Please contact Customer Service for Availability | $50.50 |
5 g | Please contact Customer Service for Availability | $205.00 |
10 g | Please contact Customer Service for Availability | $303.00 |
25 g | Please contact Customer Service for Availability | $598.00 |
100 g | Please contact Customer Service for Availability | $1,820.00 |
About This Item
Empirical Formula (Hill Notation):
C21H30O5
CAS Number:
Molecular Weight:
362.46
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
51111800
EC Number:
200-020-1
MDL number:
Beilstein/REAXYS Number:
1354819
Assay:
≥98% (HPLC)
Form:
powder
Product Name
Hydrocortisone, ≥98% (HPLC)
biological source
synthetic (organic)
Quality Segment
sterility
non-sterile
assay
≥98% (HPLC)
form
powder
mp
211-214 °C (lit.)
solubility
water: 0.28 g/L at 25 °C
application(s)
cell analysis
shipped in
ambient
storage temp.
room temp
SMILES string
[H][C@@]12CCC3=CC(=O)CC[C@]3(C)[C@@]1([H])[C@@H](O)C[C@@]4(C)[C@@]2([H])CC[C@]4(O)C(=O)CO
InChI
1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
InChI key
JYGXADMDTFJGBT-VWUMJDOOSA-N
Gene Information
human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)
Biochem/physiol Actions
Primary glucocorticoid secreted by the adrenal cortex.
Primary glucocorticoid secreted by the adrenal cortex. It has three times the anti-inflammatory potency of corticosterone but much lower Na2+ retention potency.
Features and Benefits
This compound is featured on the Nuclear Receptors (Steroids) page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.
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This Item | |||
|---|---|---|---|
| description ≥98% (HPLC) | description meets USP testing specifications | description European Pharmacopoeia (EP) Reference Standard | description United States Pharmacopeia (USP) Reference Standard |
| form powder | form powder | form - | form - |
| assay ≥98% (HPLC) | assay 97.0-102.0% dry basis | assay - | assay - |
| Quality Level 300 | Quality Level 300 | Quality Level - | Quality Level - |
| storage temp. room temp | storage temp. - | storage temp. 2-8°C | storage temp. - |
| solubility water: 0.28 g/L at 25 °C | solubility water: soluble 0.28 g/L at 25 °C | solubility - | solubility - |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
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signalword
Danger
hcodes
Hazard Classifications
Repr. 1A - STOT RE 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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Is it okay to reconstitute this product in absolute ethanol with 3.625 mg of powder to make a 10 mM concentration stock? I want to add this to the culture medium for the working concentration 1uM.
1 answer-
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What is the shelf life of reconstituted product at 4C, -20C, and -80C?
1 answer-
The stability of this product in solution after reconstitution has not been specifically determined. However, it is recommended that solutions be stored in working aliquots at −20°C. This practice is considered optimal for maintaining stability, but no exact shelf life duration is provided after reconstitution.
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