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H4777

Heparan sulfate proteoglycan

≥400 μg/mL glycosaminoglycan

Synonym(s):

HSPG

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0.1 mg
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$859.00

About This Item

UNSPSC Code:
12352202
NACRES:
NA.75
MDL number:
MFCD00283416
Biological source:
mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
≥400 μg/mL glycosaminoglycan
Mol wt:
≥400 kDa

$859.00

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biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

Quality Segment

description

For cell culture use., Isolated from basement membrane of Engelbreth-Holm-Swarm mouse sarcoma., Solution in 50 mM Tris HCl, 150 mM NaCl, 1 mM EDTA, 0.1 mM PMSF, pH 7.4, containing ≥400 μg protein per ml.

form

solution

mol wt

≥400 kDa

packaging

pkg of 0.1 mg

concentration

≥400 μg/mL glycosaminoglycan

technique(s)

cell culture | mammalian: suitable

impurities

≥100 μg/mL Uronic acid

NCBI accession no.

UniProt accession no.

binding specificity

Peptide Source: Collagen Type IV
, Peptide Source: Fibronectin
, Peptide Source: Laminin

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Hspg2(15530)

General description

Heparan sulfate proteoglycan (HSPG), a glycoprotein, is an integral part of the basement membrane. It is composed of a core protein covalently bound to heparan sulfate chains. There are three groups of HSPGs: membrane HSPGs, secreted extracellular matrix HSPGs, and the secretory vesicle proteoglycan.

Application

Heparan sulfate proteoglycan has been used:

  • in phosphate buffered saline (PBS) and added to fresh citrated blood samples to achieve trauma-equivalent concentrations in a research study
  • solid phase binding assay
  • for fabrication of extracellular matrix (ECM) microarray slides

Biochem/physiol Actions

Heparan sulfate proteoglycans (HSPGs) have multiple functions in various biological processes. They contribute to basement membrane structure and provide a matrix for cell migration. HSPGs in secretory vesicles, such as serglycin, play a role in packaging granular contents, maintaining proteases activity, and regulating biological activities after secretion. HSPGs can bind and protect cytokines, growth factors, and chemokines, facilitating the formation of gradients essential for cell specification and leukocyte recruitment. They act as receptors for proteases and protease inhibitors, regulate cell-ECM attachment, cell-cell interactions, and cell motility, and can cooperate with growth factor receptors. HSPGs also function as endocytic receptors for ligand clearance and are involved in lipoprotein metabolism and morphogen gradient formation. It also acts as a docking molecule for matrilysin and other matrix metalloproteinases. Heparan Sulfate promotes the attachment of cells in culture and induces high-affinity binding of FGF-basic to cells deficient in heparin sulfate and soluble FGF receptors at a concentration of 10-100 ng/mL.

Preparation Note

This product is isolated from the basement membrane of Engelbreth-Holm-Swarm mouse sarcoma in a solution containing 50 mM Tris HCl at pH 7.4, 150 mM NaCl, 1 mM EDTA, and 0.1 mM PMSF.

Other Notes

Heparan sulfate proteoglycan is a large biomolecule composed of a core protein covalently bound to heparin sulfate chains that is an integral part of the basement membrane. The number of polysaccharide chains and the size of the core protein are variable according to the source.

Disclaimer

It is recommended to store this product at 20°C, where it will retain its function for 3 years

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This Item
C054311243217001MAB1948P
Heparan sulfate proteoglycan ≥400 μg/mL glycosaminoglycan

Sigma-Aldrich

H4777

Heparan sulfate proteoglycan

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

Sigma-Aldrich

C0543

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Laminin from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

Roche

11243217001

Laminin

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone A7L6 clone A7L6, from rat

Sigma-Aldrich

MAB1948P

Anti-Heparan Sulfate Proteoglycan (Perlecan) Antibody, clone A7L6

description

≥400 μg/mL glycosaminoglycan

description

Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

description

from mouse Engelbreth-Holm-Swarm (EHS) sarcoma

description

clone A7L6, from rat

biological source

mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane)

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

biological source

mouse (Engelbreth-Holm-Swarm (EHS) sarcoma)

biological source

rat

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

western blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable

concentration

≥400 μg/mL glycosaminoglycan

concentration

-

concentration

-

concentration

-

Quality Level

300

Quality Level

200

Quality Level

-

Quality Level

100

mol wt

≥400 kDa

mol wt

-

mol wt

900 kDa

mol wt

-

form

solution

form

lyophilized powder

form

solution

form

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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