C0543

Sigma-Aldrich

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):
EHS-collagen
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

sterility

non-sterile

type

Type IV (Miller)

product line

BioReagent

form

lyophilized powder

packaging

glass bottle of 1 vial

application(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

The substrate derived from engelbreth-holm-swarm murine sarcoma is laminin rich basement membrane. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. Type 4 collagen is a major component of basement membrane.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Recommended for use as a cell culture substratum at 6-10 μg/cm2.
Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in adhesion assays.

Packaging

1 vial in glass bottle

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Other Notes

Native collagen

Quantity

0.75 mg/vial

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Evaluation of cell binding activities of Leptospira ECM adhesins.
Robbins G T, et al.
PLoS Neglected Tropical Diseases, 9(4), e0003712-e0003712 (2015)
Cell Motility in Cancer Invasion and Metastasis (2006)
Mimicking the Extracellular Matrix: The Intersection of Matrix Biology and Biomaterials (2015)
Gels Handbook: Fundamentals, Properties, Applications null
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to...
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