MilliporeSigma
All Photos(3)

C0543

Sigma-Aldrich

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

Synonym(s):
EHS-collagen
CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

biological source

mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)

Quality Level

type

Type IV (Miller)

product line

BioReagent

form

lyophilized powder

packaging

glass bottle of 1 vial

technique(s)

cell culture | mammalian: suitable

surface coverage

6‑10 μg/cm2

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
L2020C674511179179001
Collagen from rat tail tendon

Roche

11179179001

Collagen

form

lyophilized powder

form

aqueous solution

form

solution

form

lyophilized (clear, colorless solution after reconstitution)

packaging

glass bottle of 1 vial

packaging

pkg of 1 mg

packaging

pkg of 1 mL

packaging

pkg of 30 mg

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)

solubility

-

solubility

-

solubility

-

UniProt accession no.

B1AVK5, P02463, P08122, Q63ZW6, Q9QZS0

UniProt accession no.

P02468, P02469, P19137, Q61092

UniProt accession no.

P02462

UniProt accession no.

P02454

General description

The substrate derived from engelbreth-holm-swarm murine sarcoma is laminin rich basement membrane. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. Type 4 collagen is a major component of basement membrane.
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in adhesion assays.
Recommended for use as a cell culture substratum at 6-10 μg/cm2.

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Other Notes

Native collagen

Quantity

0.75 mg/vial

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 6

1 of 6

Mimicking the Extracellular Matrix: The Intersection of Matrix Biology and Biomaterials (2015)
Cell Motility in Cancer Invasion and Metastasis (2006)
Evaluation of cell binding activities of Leptospira ECM adhesins.
Robbins G T, et al.
PLoS Neglected Tropical Diseases, 9(4), e0003712-e0003712 (2015)
Gels Handbook: Fundamentals, Properties, Applications null
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to

Articles

3D Cell Culture Tools and Techniques

3D cell culture overview. Learn about 2D vs 3D cell culture, advantages of 3D cell culture, and techniques available to develop 3D cell models

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service