This product is lyophilized from a solution which is 75 mM NaCl/ 5mM NaH2PO4. For assay purposes, this product should be reconstituted to a concentration of 75-100 Sigma units/mL in 20 mM Tris-HCl, pH 7.5, containing 0.1 mg/mL BSA and 4 mM CaCl2. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/408/920/h8891-pis-ms.pdf
H8891
Heparinase III from Flavobacterium heparinum
Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA)
Synonym(s):
Heparin Lyase III, Heparitinase I, Heparitinase from Flavobacterium heparinum
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| Size/SKU | Availability | Price |
|---|---|---|
5 units | Available to ship TODAYfromMILWAUKEE | $288.00 |
10 units | Available to ship TODAYfromMILWAUKEE | $496.00 |
50 units | Available to ship TODAYfromMILWAUKEE | $1,970.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥30 units/mg protein (enzyme + BSA)
Biological source:
bacterial (Flavobacterium heparinum)
$288.00
Available to ship TODAYDetails
biological source
bacterial (Flavobacterium heparinum)
Quality Segment
conjugate
conjugate (Glucosaminoglycan)
form
lyophilized powder
specific activity
≥30 units/mg protein (enzyme + BSA)
mol wt
70.8 kDa
application(s)
diagnostic assay manufacturing
storage temp.
−20°C
Application
Heparinase III from Flavobacterium heparinum has been used to cleave specific GAGs. It has been used to study its effect on the electrophoretic mobility of the protein reacting with the anti-aFGF (acidic fibroblast growth factor) antibody.
Biochem/physiol Actions
Heparin-degrading lyase that recognizes heparin sulfate proteoglycan as its primary substrate.
Heparinase III prevents the formation of new blood vessels in vivo. It specifically degrades heparin sulphate at the HNY-I and HNY,6X-G2 links. Unlike other heparinise, heparinase III does not contain the amino acid cysteine. It contains 13 histidine residues, which is responsible for its catalytic action. This enzyme is not active towards heparin or low molecular weight heparins.
Other Notes
One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units. Package sizes are sold in Sigma units.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
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This Item | |||
|---|---|---|---|
| description Lyophilized powder stabilized with approx. 25% (w/w) bovine serum albumin, ≥30 units/mg protein (enzyme + BSA) | description Lyophilized powder stabilized with approx. 25% bovine serum albumin, ≥200 units/mg protein (enzyme + BSA) | description Lyophilized powder stabilized with approx. 25% bovine serum albumin, lyophilized powder, ≥100 units/mg protein (enzyme + BSA) | description lyophilized powder, stabilized with ∼ 25% (w/w) bovine serum albumin, ≥200 unit/mg protein (enzyme + BSA) |
| biological source bacterial (Flavobacterium heparinum) | biological source - | biological source bacterial (Flavobacterium heparinum) | biological source bacterial (Flavobacterium heparinum) |
| specific activity ≥30 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein (enzyme + BSA) | specific activity ≥100 units/mg protein (enzyme + BSA) | specific activity ≥200 units/mg protein |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) - | application(s) - |
| form lyophilized powder | form lyophilized solid | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 70.8 kDa | mol wt 42.8 kDa | mol wt 84.1 kDa | mol wt - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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¿En qué se resuspende la enzima y qué buffer se utiliza para la reacción?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Does Product No. H8891, Heparinase III, contain calcium?
1 answer-
This product is lyophilized from a solution which is 75 mM NaCl/ 5mM NaH2PO4. We have no reason to believe that calcium ions are present.
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Is Product No. H8891, Heparinase III sold based on Sigma Units or International Units?
1 answer-
This material is sold based on Sigma Units. One Sigma Unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 7.5 at 25 °C. One International Unit (I.U.) is equivalent to approx. 600 Sigma units.
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