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H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

lyophilized powder, ≥200 units/mg protein

Synonym(s):

(R)-3-Hydroxybutanoate:NAD+ oxidoreductase, 3-HBDH

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25 units
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$433.00
50 units
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$832.00
100 units
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$1,300.00

About This Item

CAS Number:
9028-38-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
1.1.1.30 (BRENDA, IUBMB)
MDL number:
MFCD00130633
Specific activity:
≥200 units/mg protein
Biological source:
bacterial (Pseudomonas lemoignei)

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biological source

bacterial (Pseudomonas lemoignei)

Quality Segment

form

lyophilized powder

specific activity

≥200 units/mg protein

purified by

chromatography

foreign activity

lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%

storage temp.

−20°C

General description

D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).
β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.

Physical form

lyophilized powder containing sucrose, β-NAD and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

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This Item
H8509H1506G6637
β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei lyophilized powder, ≥200 units/mg protein

Sigma-Aldrich

H9408

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides Type V, lyophilized powder, 250-750 units/mg protein

Sigma-Aldrich

H8509

β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni ≥15 units/mg protein

Sigma-Aldrich

H1506

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

β-Galactose Dehydrogenase from Pseudomonas fluorescens recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

Sigma-Aldrich

G6637

β-Galactose Dehydrogenase from Pseudomonas fluorescens

description

lyophilized powder, ≥200 units/mg protein

description

Type V, lyophilized powder, 250-750 units/mg protein

description

≥15 units/mg protein

description

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

biological source

bacterial (Pseudomonas lemoignei)

biological source

-

biological source

bacterial (Pseudomonas testosteroni)

biological source

Pseudomonas fluorescens

specific activity

≥200 units/mg protein

specific activity

250-750 units/mg protein

specific activity

≥15 units/mg protein

specific activity

≥50 units/mg protein (biuret)

form

lyophilized powder

form

lyophilized powder

form

lyophilized solid

form

ammonium sulfate suspension

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

purified by

chromatography

purified by

chromatography

purified by

chromatography

purified by

-

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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