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β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei

Name: β-Hydroxybutyrate Dehydrogenase from <I>Pseudomonas lemoignei</I>
Brand: SIGMA

lyophilized powder, ≥200 units/mg protein

Synonym(s):

(R)-3-Hydroxybutanoate:NAD⁺ oxidoreductase, 3-HBDH

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About This Item

CAS Number:

9028-38-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

1.1.1.30 (BRENDA, IUBMB)

MDL number:

MFCD00130633

Specific activity:

≥200 units/mg protein

Biological source:

bacterial (Pseudomonas lemoignei)

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Properties

biological source

bacterial (Pseudomonas lemoignei)

Quality Segment

200

form

lyophilized powder

specific activity

≥200 units/mg protein

purified by

chromatography

foreign activity

lactic dehydrogenase ≤0.05%, malic dehydrogenase ≤0.1%

storage temp.

−20°C

Description

General description

D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme.

Application

Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate.

Biochem/physiol Actions

In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).

In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate.

Physical form

lyophilized powder containing sucrose, β-NAD and Tris buffer salts

Other Notes

One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.

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This Item	H8509	L3888	H1506
Sigma-Aldrich H9408 β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei	Sigma-Aldrich H8509 β-Hydroxybutyrate Dehydrogenase from Rhodopseudomonas sphaeroides	Sigma-Aldrich L3888 D-Lactic Dehydrogenase from Lactobacillus leichmannii	Sigma-Aldrich H1506 3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni
description lyophilized powder, ≥200 units/mg protein	description Type V, lyophilized powder, 250-750 units/mg protein	description lyophilized powder, 150-500 units/mg protein	description ≥15 units/mg protein
biological source bacterial (Pseudomonas lemoignei)	biological source -	biological source bacterial (Lactobacillus leichmannii)	biological source bacterial (Pseudomonas testosteroni)
specific activity ≥200 units/mg protein	specific activity 250-750 units/mg protein	specific activity 150-500 units/mg protein	specific activity ≥15 units/mg protein
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized solid
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
purified by chromatography	purified by chromatography	purified by -	purified by chromatography

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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