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I1284

Isopropyl β-D-thiogalactopyranoside solution

ReadyMade IPTG solution for Blue-white screening

Synonym(s):

IPTG solution

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Size/SKUAvailabilityPrice
5 mL

Estimated to ship onOctober 21, 2026fromMILWAUKEE

$97.30

About This Item

Empirical Formula (Hill Notation):
C9H18O5S
CAS Number:
367-93-1
Molecular Weight:
238.30
UNSPSC Code:
12352200
NACRES:
NA.52
PubChem Substance ID:
24895928
MDL number:
MFCD00063273
Beilstein/REAXYS Number:
4631
Form:
liquid

$97.30

Estimated to ship onOctober 21, 2026Details

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grade

Molecular Biology

Quality Segment

200

description

non-ionic

form

liquid

mol wt

238.3

shipped in

dry ice

storage temp.

−20°C

SMILES string

CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

General description

Isopropyl thiogalactose (IPTG) is a sugar derivative widely used for the induction of the expression of recombinant proteins in E. coli. This expression system requires that the gene of interest is cloned in plasmids containing the Lac promoter. IPTG Ready Made is a 0.2 μm filter sterilized solution of 200 mM IPTG. This reagent remains liquid at its storage temperature (-20 °C), thus avoiding freeze-thaw cycles and providing a user-friendly solution.

Application

Isopropyl β-D-thiogalactopyranoside solution is:
  • used as an inducer for the expression of single domain antibodies(sdAbs) in E coli and bioactive peptides
  • used in its half-life and decay studies
  • suitable for induction of recombinant protein expression in systems controlled by the lac repressor

Biochem/physiol Actions

Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β--galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product. Since Isopropyl β-D-thiogalactopyranoside cannot be metabolized by E. coli its concentration remains constant. Therefore, it is not a variable in the experiment.
Isopropyl β-D-thiogalactopyranoside binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner. This binding allows the transcription of genes in the lac operon, such as the gene coding for β-galactosidase. The β-galactosidase gene is transcribed and monosaccharides are the product.

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This Item
PHG0010420291I6758
Isopropyl β-D-thiogalactopyranoside solution ReadyMade IPTG solution for Blue-white screening

Sigma-Aldrich

I1284

Isopropyl β-D-thiogalactopyranoside solution

Isopropyl β-D-1-thiogalactopyranoside

SAFC

PHG0010

Isopropyl β-D-1-thiogalactopyranoside

IPTG, Animal-Free, High Purity Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.

Millipore

420291

IPTG, Animal-Free, High Purity

IPTG ≥99% (TLC), ≤0.1% Dioxane

Sigma-Aldrich

I6758

IPTG

description

ReadyMade IPTG solution for Blue-white screening

description

-

description

Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. Contains no animal-derived products.

description

≥99% (TLC), ≤0.1% Dioxane

form

liquid

form

-

form

solid

form

powder

Quality Level

200

Quality Level

300

Quality Level

100

Quality Level

200

mol wt

238.3

mol wt

-

mol wt

-

mol wt

-

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

dry ice

shipped in

-

shipped in

ambient

shipped in

-

grade

Molecular Biology

grade

-

grade

-

grade

-

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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