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Isocitric Dehydrogenase (NADP) from porcine heart

Name: Isocitric Dehydrogenase (NADP) from porcine heart
Brand: SIGMA
Price: 130 USD

Type IV, buffered aqueous glycerol solution, 3-20 units/mg protein

Synonym(s):

threo-D_s-Isocitrate: NADP⁺ oxidoreductase, ICDH, IDH

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Pack Size	Availability	Price
25 mg	Check Cart for Availability	$130.00
100 mg	Check Cart for Availability	$427.00
250 mg	Check Cart for Availability	$877.00
500 mg	Check Cart for Availability	$1,470.00

About This Item

CAS Number:

9028-48-2

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

200-289-5

MDL number:

MFCD00131420

EC Number:

1.1.1.42 (BRENDA, IUBMB)

Specific activity:

3-20 units/mg protein

$130.00

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type

Type IV

Quality Level

200

form

buffered aqueous glycerol solution

quality

≤ 0.2% of main activity is obtained with NAD as cofactor.

specific activity

3-20 units/mg protein

composition

Protein biuret

shipped in

wet ice

storage temp.

2-8°C

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1 of 1

This Item	I1877	M2634	SAB2501739
Sigma-Aldrich I2002 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich I1877 Isocitric Dehydrogenase (NADP) from porcine heart	Sigma-Aldrich M2634 Malic Dehydrogenase from porcine heart	Sigma-Aldrich SAB2501739 Anti-IDH3G antibody produced in goat
specific activity 3-20 units/mg protein	specific activity 0.5-3.0 unit/mg solid (plus numerous enzyme activities associated with porcine heart)	specific activity 600-1000 units/mg protein (biuret)	specific activity -
form buffered aqueous glycerol solution	form solid	form buffered aqueous glycerol solution	form buffered aqueous solution
shipped in wet ice	shipped in -	shipped in wet ice	shipped in wet ice
storage temp. 2-8°C	storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 100
composition Protein biuret	composition -	composition -	composition -

Application

Isocitric dehydrogenase (IDH) has been used:

for in vitro activity assay to study the effects of thorium on citric acid cycle enzymes in the mitochondrial respiration pathway[1]
as a standard to measure the impact of temperature on nicotinamide diphosphate (NADP)-IDH activity
in isocitrate assay[2]

Biochem/physiol Actions

Isocitrate dehydrogenase (IDH) is a homodimeric enzyme and participates in several processes such as adapting to hypoxia, histone demethylation, and DNA modification. This enzyme has three isoforms like IDH₁, IDH₂, and IDH₃.[3]

Physical form

Solution in 50% glycerol in EDTA buffer salts, pH 6.0

Other Notes

One unit will convert 1.0 μmole of isocitrate to α-ketoglutarate per min at pH 7.4 at 37 °C.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

ppe

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Iron-sulfur cluster deficiency can be sensed by IRP2 and regulates iron homeostasis and sensitivity to ferroptosis independent of IRP1 and FBXL5.

Erdem M Terzi et al.

Science advances, 7(22) (2021-05-28)

Intracellular iron levels are strictly regulated to support homeostasis and avoid iron-mediated ROS production. Loss of iron-sulfur cluster (ISC) synthesis can increase iron loading and promote cell death by ferroptosis. Iron-responsive element-binding proteins IRP1 and IRP2 posttranscriptionally regulate iron homeostasis.

Icksoo Lee et al.

Biochimica et biophysica acta, 1802(2), 275-283 (2009-10-20)

Noonan syndrome (NS) is an autosomal dominant disorder, and a main feature is congenital heart malformation. About 50% of cases are caused by gain-of-function mutations in the tyrosine phosphatase SHP2/PTPN11, a downstream regulator of ERK/MAPK. Recently it was reported that

Interactions between transhydrogenase and thio-nicotinamide Analogues of NAD(H) and NADP(H) underline the importance of nucleotide conformational changes in coupling to proton translocation.

Avtar Singh et al.

The Journal of biological chemistry, 278(35), 33208-33216 (2003-06-07)

Transhydrogenase couples the reduction of NADP+ by NADH to inward proton translocation across mitochondrial and bacterial membranes. The coupling reactions occur within the protein by long distance conformational changes. In intact transhydrogenase and in complexes formed from the isolated, nucleotide-binding

Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.

M Ryan Smith et al.

Redox biology, 8, 136-148 (2016-01-18)

Many cancer cells follow an aberrant metabolic program to maintain energy for rapid cell proliferation. Metabolic reprogramming often involves the upregulation of glutaminolysis to generate reducing equivalents for the electron transport chain and amino acids for protein synthesis. Critical enzymes

Acidic pH Is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling.

Sergiy M Nadtochiy et al.

The Journal of biological chemistry, 291(38), 20188-20197 (2016-08-12)

2-Hydroxyglutarate (2-HG) is an important epigenetic regulator, with potential roles in cancer and stem cell biology. The d-(R)-enantiomer (d-2-HG) is an oncometabolite generated from α-ketoglutarate (α-KG) by mutant isocitrate dehydrogenase, whereas l-(S)-2-HG is generated by lactate dehydrogenase and malate dehydrogenase

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Global Trade Item Number

SKU	GTIN
I2002-250MG	04061832730431
I2002-25MG	04061832730448
I2002-5G-A	04061832490205
I2002-100MG	04061833848234
I2002-500MG	04061832730455
I2002-25G-A	04061833848241

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