This antibody is specific for an epitope expressed in the Fc region of human IgG1. It does not cross-react is other human IgG subclasses. It has not been tested for cross-reactivity with other species.
I2513
Monoclonal Anti-Human IgG1 antibody produced in mouse
clone 8c/6-39, ascites fluid
Synonym(s):
Monoclonal Anti-Human IgG1 (Fc specific)
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| Size/SKU | Availability | Price |
|---|---|---|
0.2 mL | Available to ship TODAYfromMILWAUKEE | $256.00 |
0.5 mL | Available to ship TODAYfromMILWAUKEE | $509.00 |
About This Item
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
8c/6-39, monoclonal
Application:
ELISA (i)
Citations:
20
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
secondary antibodies
clone
8c/6-39, monoclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:400
isotype
IgG2a
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Human IgG consists of four subclasses (1-4) that can be recognized by antigen differences in their heavy chains. They constitute approximately 65, 25, 6 and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.
The IgG subclass may be preferentially produced in response to different antigens. For instance, antipolysaccharide responses are mainly of the IgG2 subclass, while protein antigens give rise to IgG1 and IgG3 antibodies. Lipopolysaccahrides stimulate an IgG2 response in PBL′s and an IgG1 response in the spleen. Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes. Serum IgG subclass deficiencies have been recorded for different patient groups. For example, IgG2 and IgG4 deficiency is associated with IgA deficiency as found in patients of ataxia telangiextasia. Low IgG2 levels were found in patients with SLE and juvenile diabetes melitus. A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis. Examination of the distribution pattern of IgG subclasses in different types of diseases may provide insight into the immunological processes involved and may assist in the diagnosis of various disorders.
The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.
Application
Monoclonal Anti-Human IgG1 antibody produced in mouse has been used in:
- direct hemagglutination(HA)
- hemagglutination inhibition(HAI) assays
- enzyme linked immunosorbent assay (ELISA)
- immunofluorometric assay (IFMA)
- immunofluorescence
- cell-based assays for the detection of IgG subclasses of acetylcholine receptor (AChR) antibodies
Mouse monoclonal clone clone 8c/6-39 anti-Human IgG1 antibody may be used for the identification of human IgG1 subclass by means of various immunoassays and direct hemagglutination (HA) and hemagglutination inhibition (HAI) assays, enzyme linked immunosorbent assay (ELISA), immunofluorometric assay (IFMA) and detection of cytoplasmic IgG. The antibody may be useful in the analysis of the distribution of IgG subclasses in various diseases.
Biochem/physiol Actions
Human IgG consists of four subclasses (1-4) that can be recognized.
Human IgG1 is the predominant subclass of in vivo and in vitro produced anti-tetanus toxoid antibodies. Only IgG1 and IgG3 are capable of adherence to mononuclear phagocytes via Fc receptors (FcR). A disproportionate elevation of IgG1 has also been found in the cerebral spinal fluid of patients with multiple sclerosis.
Physical form
The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.
Preparation Note
For continuous use, store at 0-5 °C. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone 8c/6-39, ascites fluid | description clone HP-6002, ascites fluid | description clone 8C/6-39, purified from hybridoma cell culture | description whole antiserum |
| conjugate unconjugated | conjugate unconjugated | conjugate - | conjugate unconjugated |
| antibody form ascites fluid | antibody form ascites fluid | antibody form purified from hybridoma cell culture | antibody form whole antiserum |
| technique(s) indirect ELISA: 1:400 | technique(s) indirect ELISA: 1:2,000 | technique(s) indirect ELISA: 3-6 μg/mL using Human IgG1 myeloma protein for coating. | technique(s) immunoelectrophoresis: suitable, direct immunofluorescence: 1:160 using human peripheral blood lymphocytes, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160 |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| isotype IgG2a | isotype IgG1 | isotype IgG2a | isotype - |
| clone 8c/6-39, monoclonal | clone HP-6002, monoclonal | clone 8C/6-39, monoclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hi, I was wondering if this product I2513 has been tested for crossreactivity with other species, specifically with mouse IgG. Regards, Jean
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