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I7385

Anti-Human IgG4 antibody, Mouse monoclonal

clone HP-6025, purified from hybridoma cell culture

Synonym(s):

Anti-Human IgG4 antibody, Mouse monoclonal

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100 μL

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$430.00
0.2 mL
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$547.00

About This Item

NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
HP-6025, monoclonal
Application:
ELISA (i), IF
Citations:
14

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biological source

mouse

Quality Segment

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6025, monoclonal

form

(Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide)

contains

15 mM sodium azide

technique(s)

immunofluorescence: suitable, indirect ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines. Unlike other immunoglobulin G is rare and constitutes to only 3-4 percent of the total IgG in the serum.

Application

Monoclonal Anti-HuIgG4 man antibody produced in mouse has been used in ELISA and immunofluorescence.
The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differing from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4. Increased levels of IgG4 is observed in atopic dermatitis, parasitic diseases, pemphigus vulgaris and pemphigus foliaceus.
Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Anti-Human IgG4 antibody, Mouse monoclonal clone HP-6025, purified from hybridoma cell culture

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Anti-Human IgG2−Biotin antibody, Mouse monoclonal clone HP-6014, purified from hybridoma cell culture

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Anti-Human IgG2−Biotin antibody, Mouse monoclonal

description

clone HP-6025, purified from hybridoma cell culture

description

clone HP-6017, ascites fluid

description

clone HP-6025, purified from hybridoma cell culture

description

clone HP-6014, purified from hybridoma cell culture

conjugate

unconjugated

conjugate

unconjugated

conjugate

FITC conjugate

conjugate

biotin conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

technique(s)

immunofluorescence: suitable, indirect ELISA: 1:5,000

technique(s)

indirect ELISA: 1:3,000

technique(s)

dot immunobinding: 1:64 using using a 4-8 μg dot of human IgG4

technique(s)

direct ELISA: 1:30,000

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

clone

HP-6025, monoclonal

clone

HP-6017, monoclonal

clone

HP-6025, monoclonal

clone

HP-6014, monoclonal

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Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

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