The molecular weight of this material has not been determined. Human serum IgM is a pentamer and has 10 heavy chains and 10 light chains, connected by a J chain. The total molecular weight is expected to be approximately 970kDa. The unmodified human IgM subunit weight of the m chain is 75 kDa and the k chain is 22 kDa.
I8260
IgM from human serum
reagent grade, ~95% (HPLC), buffered aqueous solution
Synonym(s):
Human IgM
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| Size/SKU | Availability | Price |
|---|---|---|
1 mg | Available to ship TODAYfromMILWAUKEE | $790.00 |
5 mg | Available to ship TODAYfromMILWAUKEE | $790.00 |
10 mg | Available to ship TODAYfromMILWAUKEE | $1,320.00 |
25 mg | Available to ship TODAYfromMILWAUKEE | $2,820.00 |
About This Item
Conjugate:
unconjugated
Species reactivity:
-
Application:
—
Citations:
76
conjugate
unconjugated
Quality Segment
grade
reagent grade
assay
~95% (HPLC)
form
buffered aqueous solution
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
General description
There are five immunoglobulin classes in humans. Out of these, immunoglobulin M (IgM) is a high molecular weight protein that has five or six subunits. IgM monomers are made of two heavy chains and two light chains connected by a disulfide bond. Human serum shows low concentration of IgM monomers. It has a high carbohydrate content of about 12%. It is the first immunoglobulin produced by neonates.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.
IgM antibodies are present as pentamers in the serum and are produced in response to antigens.
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.
Application
Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml) and dot blot assay. Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays.
IgM from human serum has been used
IgM from human serum has been used
- in ELISA inhibition assay
- in immunoblotting
- as MW (molecular weight) standards
- in IgM binding assay
- to confirm the depletion of immunoglobulins
Biochem/physiol Actions
Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions.
Physical form
Solution in 0.05 M Tris-HCl, 0.2 M sodium chloride, pH 8.0, containing 15 mM sodium azide.
Preparation Note
Store at 2-8 °C. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| description reagent grade, ~95% (HPLC), buffered aqueous solution | description reagent grade, ≥95% (SDS-PAGE or HPLC), buffered aqueous solution | description reagent grade, ≥95% (HPLC), buffered aqueous solution | description clone MOPC 104E, purified immunoglobulin, buffered aqueous solution |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in dry ice |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| assay ~95% (HPLC) | assay ≥95% (SDS-PAGE or HPLC) | assay ≥95% (HPLC) | assay - |
| Quality Level 300 | Quality Level 300 | Quality Level 300 | Quality Level 200 |
Storage Class
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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What is the molecular weight of the IgM antibody from human serum with the product code I8260?
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