This product can be aliquoted and stored at -60°C in working aliquots for long-term preservation. Repeated freeze-thaw cycles should be avoided to maintain activity. Although specific -60°C stability data aren’t available, this storage approach is generally used for long-term preservation of such products.
I8885
Anti-Human IgG (Fc specific) antibody produced in goat
whole antiserum
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| Size/SKU | Availability | Price |
|---|---|---|
2 mL | Available to ship TODAYfromMILWAUKEE | $160.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA (i), QPA
Citations:
4
biological source
goat
Quality Segment
conjugate
unconjugated
antibody form
whole antiserum
antibody product type
secondary antibodies
clone
polyclonal
contains
15 mM sodium azide
technique(s)
indirect ELISA: 1:50,000, quantitative precipitin assay: 4.0-7.0 mg/mL
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Immunoglobulin G (IgG), a widely expressed serum antibody, has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein, usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 % of the total immunoglobulin consists of IgG.
Application
Biochem/physiol Actions
Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III. It provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Specificity for the Fc fragment of human IgG is determined by immunoelectrophoresis (IEP) versus normal human serum and Fc fragment of human IgG. No reactivity is observed versus purified human IgA, IgG (Fab fragment), IgM, Bence Jones kappa and Bence Jones lambda myeloma proteins.
Physical form
Goat Anti-Human IgG is supplied as a liquid containing 0.1% sodium azide (see MSDS) as a preservative.
Preparation Note
For continuous use, store at 2-8°C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description whole antiserum | description affinity isolated antibody | description whole antiserum | description whole antiserum |
| antibody form whole antiserum | antibody form affinity isolated antibody | antibody form whole antiserum | antibody form whole antiserum |
| biological source goat | biological source goat | biological source goat | biological source goat |
| technique(s) quantitative precipitin assay: 4.0-7.0 mg/mL, indirect ELISA: 1:50,000 | technique(s) direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, dot blot: 1:100,000 (chemiluminescent) | technique(s) quantitative precipitin assay: 4.0 mg/mL, indirect ELISA: 1:30,000 | technique(s) indirect ELISA: 1:50,000, quantitative precipitin assay: 3.0-5.0 |
| conjugate unconjugated | conjugate peroxidase conjugate | conjugate unconjugated | conjugate unconjugated |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
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Storage Class
10 - Combustible liquids
WGK
WGK 1
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Can this product be aliquoted and stored at -60 °C for extended long-term storage?
1 answer-
Helpful?
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Hi, I wanted to know the concentration of this antibody (product I8885). I see it is in 2mL of liquid but i can't find information regarding the amount of antibody (mg) in the solution.
1 answer-
The antibody concentration of this product is lot specific and may range from 4.0 - 7.0 mg/mL. This information is reported in the Certificate of Analysis. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/i8885#product-documentationHelpful?
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