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L3892

Lectin from Triticum vulgaris (wheat)

peroxidase conjugate, lyophilized powder

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Synonym(s):

WGA, Wheat germ agglutinin

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1 mg

Available to ship TODAYfromMILWAUKEE

$300.00
2 mg

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$545.00
5 mg
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$1,030.00

About This Item

NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Assay:
≥85% protein basis (Warburg-Christian)
Biological source:
Triticum vulgaris

$300.00

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biological source

Triticum vulgaris

Quality Segment

conjugate

peroxidase conjugate

assay

≥85% protein basis (Warburg-Christian)

form

lyophilized powder

potency

<40 μg/mL agglutination activity

peroxidase activity

50-200 units/mg protein

composition

Protein, ~90% modified Warburg-Christian

storage temp.

−20°C

Application

Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains citrate buffer

Preparation Note

Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Conjugates are prepared from affinity purified lectin.

Other Notes

One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.

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L1882

Lectin from Triticum vulgaris (wheat)

description

peroxidase conjugate, lyophilized powder

description

lyophilized, ≥55% (GE), powder

description

suitable for cell culture, BioReagent, lyophilized powder

description

agarose conjugate, saline suspension

biological source

Triticum vulgaris

biological source

Triticum vulgaris

biological source

-

biological source

-

assay

≥85% protein basis (Warburg-Christian)

assay

-

assay

-

assay

-

form

lyophilized powder

form

powder

form

lyophilized powder

form

saline suspension

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

potency

<40 μg/mL agglutination activity

potency

-

potency

-

potency

-

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

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Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000578877
    0000505869
    0000083745
    0000134389
    0000083746

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