Yes, the remaining powder product is stable when stored refrigerated.
L4005
Lipopolysaccharides from Escherichia coli O55:B5
purified by trichloroacetic acid extraction
Synonym(s):
LPS
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| Size/SKU | Availability | Price |
|---|---|---|
100 mg | Available to ship TODAYfromMILWAUKEE | $322.00 $336.00 |
500 mg | Check Cart for Availability | $1,390.00 |
About This Item
biological source
Escherichia coli (O55:B5)
Quality Segment
form
lyophilized powder
purified by
trichloroacetic acid extraction
impurities
1-10% Protein (Lowry)
color
white to yellow
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
General description
This product is TCA extracted from E. coli serotype O55:B5. The source strain is CDC 1644-70. The LPS O55:B5 has been used to stimulate human peritoneal macrophages at 1 ng/ml and to stimulate equine peritoneal macrophages at 1-100 ng/ml.
Application
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
Biochem/physiol Actions
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
Preparation Note
The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
Other Notes
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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This Item | |||
|---|---|---|---|
| description purified by trichloroacetic acid extraction | description purified by ion-exchange chromatography, TLR ligand tested | description purified by phenol extraction | description FITC conjugate |
| biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O55:B5) | biological source Escherichia coli (O55:B5) |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| impurities 1-10% Protein (Lowry) | impurities <1% Protein, <1% RNA | impurities <3% Protein (Lowry) | impurities - |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral
Storage Class
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Is there any recommendation in how to store the powder if I will not prepare all the 100 mg, Is it still active to store them in the fridge ?
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