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L4025

Lyticase from Arthrobacter luteus

lyophilized powder, ≥200 units/mg solid

Synonym(s):

(1,3)-β-D-Glucan endohydrolase, 1,3-β-Glucan glucohydrolase, Bacterial lyticase, Lysing enzyme

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25000 units
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$84.60
50000 units
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$140.00
100000 units
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$276.00
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$1,280.00

About This Item

CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
≥200 units/mg solid
Biological source:
bacterial (Arthrobacter luteus)

$84.60


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biological source

bacterial (Arthrobacter luteus)

Quality Segment

form

lyophilized powder

specific activity

≥200 units/mg solid

technique(s)

cell based assay: suitable

input

plant, fungi and insect sample(s)

suitability

suitable for cell lysis

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Application

Lyticase from Arthrobacter luteus has been used:
  • as a component in spheroplasting buffer to prepare yeast nuclear extracts
  • for digestion in sample preparation for β-glucan enzymatic assay using yeast
  • to lyse cells for RNA isolation

Lyticase from Arthrobacter luteus has been used for resuspending cells to extract high-molecular weight DNA for polymerase chain reaction (PCR) fingerprint analyses.

Biochem/physiol Actions

Lyticase is a lysing enzyme that is used to extract DNA from yeast cells by inducing partial spheroplast formation. Spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast, which are difficult to disrupt because the cell walls may form capsules or resistant spores. Lyticase contains β-(1→;3)-glucan laminaripentaohydrolase along with β-(1→3)-glucanase, protease, and mannanase activities. It is useful with yeast cells such as e.g. Candida, Debaryomyces, Saccharomyces, Saccharomycopsis, Saccharomycodes, Eremothecium, and Schwanniomyces species.
Lyticase enzyme is frequently used in fungal research, particularly for species identification using polymerase chain reaction (PCR)-based techniques. It can break down β (1→3) and β (1→4) bonds between glucose units.
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.

Other Notes

For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.

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description

lyophilized powder, ≥200 units/mg solid

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lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret

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powder, ≥400 units/mg protein (Lowry)

description

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

technique(s)

cell based assay: suitable

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

specific activity

≥200 units/mg solid

specific activity

≥2,000 units/mg protein

specific activity

≥400 units/mg protein (Lowry)

specific activity

≥500 units/mg protein (biuret)

biological source

bacterial (Arthrobacter luteus)

biological source

bacterial (Arthrobacter luteus)

biological source

Bacillus sp.

biological source

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

form

lyophilized powder

form

lyophilized powder

form

powder

form

saline solution

suitability

suitable for cell lysis

suitability

suitable for cell lysis

suitability

-

suitability

-


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pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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