L6544
LuminoCt® SYBR™ Green qPCR ReadyMix™
For fast SYBR Green quantitative PCR
Synonym(s):
LuminoCt®
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
100 reactions | Check Cart for Availability | $302.00 |
500 reactions | Check Cart for Availability | $1,320.00 |
2000 reactions | Check Cart for Availability | $4,940.00 |
About This Item
$302.00
Quality Segment
form
liquid
usage
sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions
feature
dNTPs included, hotstart
technique(s)
RT-qPCR: suitable, qPCR: suitable, whole genome amplification: suitable
color
colorless
input
purified DNA
application(s)
agriculture
compatibility
Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ 5, for use with ABI 5700, for use with ABI 7000, for use with ABI 7300, for use with ABI 7500 Fast, for use with ABI 7500, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI 7900, for use with ABI StepOne, for use with ABI StepOnePlus, for use with ABI ViiA 7, for use with Bio-Rad CFX384, for use with Bio-Rad CFX96, for use with Bio-Rad MJ Chromo4, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MJ Opticon, for use with Bio-Rad MiniOpticon, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Eppendorf® Mastercycler ep realplex, for use with Illumina Eco qPCR, for use with Qiagen Corbett Rotor-Gene 3000, for use with Qiagen Corbett Rotor-Gene 6000, for use with Qiagen Corbett Rotor-Gene Q, for use with Roche LightCycler 480, for use with Strategene Mx3000P, for use with Strategene Mx3005P, for use with Strategene Mx4000
detection method
SYBR™ Green
shipped in
wet ice
storage temp.
−20°C
General description
- Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
- ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
- Kit is not compatible with qPCR instruments that utilize glass capillary tubes
Application
Features and Benefits
- Assay results in as little as 25 minutes
- Unsurpassed accuracy, precision, and sensitivity
- Virtually eliminates the need to optimize assay parameters
- Ideal for performing high-throughput, quantitative PCR applications
- The master mix allows consistency from one reaction to the next
- Reduced preparation time and reduced risk of contamination from multiple pipetting steps
- The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield
- LuminoCt® ReadyMixes require the use of small amplicons (<200bp) for optimal results
- This master mix is compatible with commercial primer sets, including TaqMan® assays
Packaging
100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL
Other Notes
- At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
- Sigma recommends designing primers and probes with Beacon Designer software
- To order primers and probes from Sigma, visit: www.sigma.com/oligos
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| description For fast SYBR Green quantitative PCR | description SYBR™ Green qPCR reagent, passive reference dye included | description For probe-based real-time PCR | description For fast probe-based quantitative PCR |
| technique(s) whole genome amplification: suitable, qPCR: suitable, RT-qPCR: suitable | technique(s) whole genome amplification: suitable, qPCR: suitable | technique(s) whole genome amplification: suitable, qPCR: suitable, RT-qPCR: suitable | technique(s) RT-qPCR: suitable, whole genome amplification: suitable |
| usage sufficient for 500 reactions, sufficient for 2000 reactions, sufficient for 100 reactions | usage sufficient for 2000 reactions, sufficient for 20 reactions, sufficient for 400 reactions | usage sufficient for 400 reactions, sufficient for 100 reactions, sufficient for 20 reactions | usage sufficient for 2000 reactions, sufficient for 500 reactions, sufficient for 100 reactions |
| input purified DNA | input purified DNA | input purified DNA | input purified DNA |
| color colorless | color colorless | color colorless | color colorless |
| compatibility for use with ABI 7700, for use with ABI 7900, for use with Qiagen Corbett Rotor-Gene Q, for use with ABI 7500, for use with ABI 7900 HT, Bio-Rad MyiQ, for use with ABI 7500 Fast, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Strategene Mx3000P, for use with Bio-Rad CFX384, Bio-Rad iCycler iQ, for use with ABI 5700, for use with ABI StepOne, for use with Qiagen Corbett Rotor-Gene 3000, for use with Illumina Eco qPCR, for use with ABI StepOnePlus, Bio-Rad iQ 5, for use with Roche LightCycler 480, for use with Strategene Mx4000, for use with ABI ViiA 7, for use with Bio-Rad MiniOpticon, for use with Strategene Mx3005P, for use with Bio-Rad CFX96, for use with ABI 7900 Fast, for use with Qiagen Corbett Rotor-Gene 6000, for use with Bio-Rad MJ Opticon 2, for use with Bio-Rad MJ Chromo4, for use with ABI 7300, for use with ABI 7000 | compatibility for use with ABI 7000, for use with ABI 7900, for use with ABI 5700, for use with ABI 7300, for use with ABI 7700, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with ABI StepOne, for use with ABI StepOnePlus | compatibility for use with Eppendorf® Mastercycler ep realplex, for use with Bio-Rad MJ Opticon 2, for use with Eppendorf® Mastercycler ep realplex2 s, for use with ABI 7900 Fast, Bio-Rad MyiQ ( ), for use with ABI 5700, Bio-Rad iQ 5, for use with Qiagen Corbett Rotor-Gene 6000, for use with Bio-Rad CFX96, for use with Strategene Mx4000, for use with ABI 7900 HT, for use with ABI 7000, for use with Qiagen Corbett Rotor-Gene 3000, for use with ABI StepOne, for use with ABI 7300, Bio-Rad iCycler iQ, for use with Bio-Rad MiniOpticon, for use with Bio-Rad CFX384, for use with ABI 7700, for use with ABI 7500 Fast, for use with Roche LightCycler 480, for use with ABI ViiA 7, for use with Bio-Rad MJ Chromo4, for use with Qiagen Corbett Rotor-Gene Q, for use with Strategene Mx3000P, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Illumina Eco qPCR, for use with ABI StepOnePlus, for use with Strategene Mx3005P | compatibility for use with Qiagen Corbett Rotor-Gene 6000, Bio-Rad MyiQ ( ), for use with ABI 7000, for use with Bio-Rad MJ Opticon 2, for use with ABI 7900 Fast, for use with ABI 7900 HT, for use with Bio-Rad MJ Chromo4, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with Qiagen Corbett Rotor-Gene 3000, Bio-Rad iCycler iQ, for use with ABI StepOnePlus, for use with ABI 7500 Fast, for use with Illumina Eco qPCR, for use with Bio-Rad CFX384, for use with Strategene Mx3005P, for use with Roche LightCycler 480, for use with Strategene Mx4000, for use with Bio-Rad CFX96, for use with ABI 7300, for use with ABI ViiA 7, for use with Eppendorf® Mastercycler ep realplex2 s, for use with Strategene Mx3000P, for use with Qiagen Corbett Rotor-Gene Q, Bio-Rad iQ 5, for use with ABI 5700, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with Bio-Rad MiniOpticon |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Kit Components Only
- 2 X
Kit Components Also Available Separately
Storage Class
10 - Combustible liquids
wgk
WGK 1
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