This product is supplied in the flavin adenine dinucleotide-bound form and is not an apoenzyme.
L9795
Lactate Oxidase from Aerococcus viridans
lyophilized powder
Synonym(s):
L-lactate monooxygenase, lactate monooxygenase, lactate oxidative decarboxylase, lactate oxygenase, lactic oxidase, lactic oxygenase
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| Size/SKU | Availability | Price |
|---|---|---|
50 units | Available to ship TODAYfromMILWAUKEE | $205.00 |
100 units | Available to ship TODAYfromMILWAUKEE | $344.00 |
500 units | Available to ship TODAYfromMILWAUKEE | $1,380.00 |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Biological source:
bacterial (Aerococcus viridans)
Quality Segment
biological source
bacterial (Aerococcus viridans)
form
lyophilized powder
mol wt
80 kDa (gel filtration)
storage temp.
−20°C
General description
Lactate oxidase (LOX) is a flavin mononucleotide (FMN)-dependent enzyme and is a member of the α-hydroxy-acid oxidase flavoenzyme family.
Application
Lactate Oxidase from Aerococcus viridans has been used as a:
- supplement in the culture medium to convert extracellular lactate to pyruvate
- coating on glass beads for electrochemical detection of lactate from a single cell by a push-pull nozzle system
- coating on multi-branched gold nanoparticles (GNPs) for the simultaneous colorimetric detection of lactate in saliva by paper-based multiplexed device
Biochem/physiol Actions
Lactate oxidase (LOX) catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide in the presence of an FMN co-factor. The enzyme aids in the determination of L-lactate indirectly by measuring the hydrogen peroxide formed in the reaction.
Other Notes
One unit is defined as the amount of enzyme which generates 1m mole of Hydrogen Peroxide per minute at 37°C under standard assay conditions.
1 of 1
This Item | |||
|---|---|---|---|
| description lyophilized powder | description lyophilized powder, ≥70 units/mg solid | description lyophilized powder, ≥35 units/mg protein (biuret) | description 50’000-150’000 CFU mean value range, certified reference material, suitable for microbiology, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland |
| biological source bacterial (Aerococcus viridans) | biological source - | biological source bacterial (Aerococcus spp.) | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt 80 kDa (gel filtration) | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 300 |
Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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Hello, is this an apo enzyme or already FMN bound?
1 answer-
Helpful?
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What is the activity of this enzyme (U/g)?
1 answer-
The minimum activity of this enzyme is 20 units per milligram. A survey of recent lots indicates a typical range of 45 - 50 units per milligram. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/l9795#product-documentationHelpful?
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Hello, I am a research chemist. I would kindly like to know the excipients present in Lactate Oxidase from Aerococcus viridans L9795. Thank you in advance.
1 answer-
This freeze-dried preparation contains the following components:
Recombinant Lactate Oxidase at 15-20%
Sucrose at 50-84%
Potassium Phosphate monobasic at 1-30%Helpful?
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