This antibody product is in mouse ascites fluid and has not been purified. The total IgG1 concentration is reported in the product Certificate of Analysis and will range from 3.0 - 8.0 mg/mL. The Quality testing included optimization of the dilution factor for usage in Western Blot. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sigma/m5546#product-documentation
M5546
Anti-c-Myc Tag Antibody
mouse monoclonal, 9E10
Synonym(s):
Anti-c-Myc
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Available to ship TODAYfromMILWAUKEE | $552.00 |
0.2 mL | Available to ship TODAYfromMILWAUKEE | $727.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Conjugate:
unconjugated
Clone:
9E10, monoclonal
Application:
ELISA (i), ICC, IHC (p), IP, WB
Citations:
193
Product Name
Monoclonal Anti-c-Myc antibody produced in mouse, clone 9E10, ascites fluid
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
9E10, monoclonal
contains
15 mM sodium azide
species reactivity
human
technique(s)
immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:100 using c-myc tagged fusion protein
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... MYC(4609)
General description
MYC (MYC proto-oncogene) is a transcriptional factor and oncoprotein. c-myc is a member of MYC gene family. It is located on human chromosome 8q24. c-Myc gene codes for basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
Monoclonal Anti-c-myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice.
c-Myc is a transcription factor which is over-expressed in a wide range of cancers.
Immunogen
synthetic peptide of the human p62c-Myc protein.
Application
Monoclonal anti-c-Myc antibody produced in mouse has been used for Western blotting and immunohistochemistry.
Monoclonal Anti-c-Myc antibody has also been used in:
Monoclonal Anti-c-Myc antibody has also been used in:
- the detection of horseradish peroxidase conjugate in phosphate buffered saline tween-20 (PBST) and 65-kDa mannoprotein (MP65) Dabs
- immunoblotting
- immunoprecipitation
- biochemical and histological studies
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Biochem/physiol Actions
Recognizes both N- terminal and C-terminal c-Myc tagged proteins.
The cellular myelocytomatosis (c-myc) oncogene plays a major role in cellular proliferation, differentiation and apoptosis. It acts as a transcriptional regulator of gene expression. MYC (MYC proto-oncogene) may be essential for cancer initiation, promotion and therapy resistance. Overexpression of MYC in DLBCL (diffuse large B-cell lymphoma) results in the poor outcome and invasive treatment, when medicated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP).
c-Myc is a regulator of the cell cycle and aids in the growth of tumors in the liver. Translocations in the gene encoding it have been shown to be linked to Burkitt lymphoma. It has been studied that c-Myc targets many protein-coding genes which enhances the oncogenic properties of c-Myc.
Physical form
The product is provided as ascites fluid with 15 mM sodium azide as a preservative.
Preparation Note
For continuous use, store at 2-8 °C for a maximum of one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description clone 9E10, ascites fluid | description clone 9E10, purified from hybridoma cell culture | description clone 9E10, purified immunoglobulin, buffered aqueous solution | description affinity isolated antibody |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human, mouse, rat |
| conjugate unconjugated | conjugate unconjugated | conjugate CY3 conjugate | conjugate unconjugated |
| Gene Information human ... MYC(4609) | Gene Information human ... MYC(4609) | Gene Information human ... MYC(4609) | Gene Information human ... MYC(4609) |
| antibody form ascites fluid | antibody form purified from hybridoma cell culture | antibody form purified immunoglobulin | antibody form affinity isolated antibody |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| clone 9E10, monoclonal | clone 9E10, monoclonal | clone 9E10, monoclonal | clone polyclonal |
Still not finding the right product?
Explore all of our products under Monoclonal Anti-c-Myc antibody produced in mouse
— or —
Try our Product Selector Tool to narrow your options
Storage Class
12 - Non Combustible Liquids
WGK
nwg
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
-
Ab 농도 문의드립니다.
1 answer-
Helpful?
-